Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

被引：56

作者：

Meyer, S. ^{[1
,2
]}

Lopez-Serrano, A. ^{[3
]}

Mitze, H. ^{[1
]}

Jakubowski, N. ^{[3
]}

Schwerdtle, T. ^{[1
,2
,4
]}

机构：

[1] Univ Potsdam, Inst Nutr Sci, Arthur Scheunert Allee 114-116, D-14558 Nuthetal, Germany

[2] NutriAct Competence Cluster Nutr Res Berlin Potsd, Nuthetal, Germany

[3] Bundesanstalt Mat Forsch & Prufung BAM, Richard Willstatter Str 11, D-12489 Berlin, Germany

[4] DFG Res Unit, Interact Essential Trace Elements Hlth & Dis Elde, FOR 2558, Nuthetal, Germany

来源：

METALLOMICS | 2018年 / 10卷 / 01期

关键词：

MASS-SPECTROMETRY; QUANTIFICATION; NANOPARTICLES;

D O I：

10.1039/c7mt00285h

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 mu M for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

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收藏

页码：73 / 76

页数：4

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