Effect of ergosta-4,6,8(14),22-tetraen-3-one (ergone) on adenine-induced chronic renal failure rat: A serum metabonomic study based on ultra performance liquid chromatography/high-sensitivity mass spectrometry coupled with MassLynx i-FIT algorithm

被引：92
作者：
Zhao, Ying-Yong [1 ]
Cheng, Xian-Long [2 ]
Cui, Ji-Hong [1 ]
Yan, Xing-Rong [1 ]
Wei, Feng [2 ]
Bai, Xu [3 ]
Lin, Rui-Chao [2 ]
机构：
[1] NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Shaanxi, Peoples R China
[2] State Food & Drug Adm, Natl Inst Food & Drug Control, Beijing 100050, Peoples R China
[3] Waters Technol Shanghai Ltd, Shanghai 201203, Peoples R China
来源：
CLINICA CHIMICA ACTA | 2012年 / 413卷 / 19-20期
关键词：
Metabonomics; Chronic renal failure; Ergosta-4.6,8(14),22-tetraen-3-one; Polyporus umbellatus; UPLC/HSMS; MassLynx i-FIT algorithm; POTENTIAL BIOMARKERS; POLYPORUS-UMBELLATUS; PHARMACOKINETICS; PLASMA; PHENYLALANINE; EXPRESSION; TOXICOLOGY; STEROIDS; KIDNEY; INJURY;
D O I：
10.1016/j.cca.2012.06.005
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Ergosta-4.6,8(14),22-tetraen-3-one (ergone) has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. We investigated the therapeutic effects and mechanism of ergone on a chronic renal failure model of rats induced by adenine. Methods: A serum metabonomic method based on the UPLC Q-TOF/MS was undertaken to explore the excretion pattern of low molecular mass metabolites. Results: Coupled with blood biochemistry and kidney histopathology results, the significant difference in metabolic profiling between adenine-induced chronic renal failure group and the ergone treated group by using pattern recognition analysis indicated that changes in global serum metabolites occurred. Some significantly changed metabolites like lysophosphatidylcholines, adenine, dopamine, creatinine, aspartic acid and phenylalanine have been found and identified. These biochemical changes in serum metabolites are related to the perturbations of amino acid metabolism and lecithin metabolism, which may be helpful to further understand the chronic renal failure and therapeutic mechanisms of ergone. Conclusion: The work shows that the metabonomic method is a valuable tool for studying the essence of chronic kidney disease and therapeutic effect mechanism of preclinical or clinical drug. (C) 2012 Elsevier B.V. All rights reserved.
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页码：1438 / 1445
页数：8
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