Quantitative Proton Nuclear Magnetic Resonance evaluation and total assignment of the capsular polysaccharide Neisseria meningitidis serogroup X

被引：16

作者：

Garrido, Raine ^{[1
]}

Puyada, Alain ^{[1
]}

Fernandez, Anais ^{[1
]}

Gonzalez, Majela ^{[1
]}

Ramirez, Ubel ^{[2
]}

Cardoso, Felix ^{[1
]}

Valdes, Yury ^{[1
]}

Gonzalez, Domingo ^{[2
]}

Fernandez, Violeta ^{[1
,2
]}

Verez, Vicente ^{[1
]}

Velez, Herman ^{[1
]}

机构：

[1] Ctr Biomol Chem, Havana, Cuba

[2] Finlay Inst, Havana, Cuba

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2012年 / 70卷

关键词：

Neisseria meningitidis; X serogroup; NMR; Assignment; Quantitative analysis; SPECTROSCOPY; OUTBREAK; H-1-NMR;

D O I：

10.1016/j.jpba.2012.07.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, V. and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional C-13 NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving C-13, H-1, and P-31 nuclei. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：295 / 300

页数：6

共 21 条

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