Isolation and characterization of 12 polymorphic microsatellite markers from ladyfish (Elops saurus Linnaeus)

被引：2

作者：

Sha, Zhen-Xia ^{[1
]}

Xing, Shi-Chao ^{[1
,2
]}

Shao, Chang-Wei ^{[1
]}

Tian, Yong-Sheng ^{[1
]}

Liao, Xiao-Lin ^{[1
]}

Chen, Song-Lin ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 06期

关键词：

Ladyfish; Elops saurus Linnaeus; Enriched genomic library; SSR; Microsatellites; DINUCLEOTIDE;

D O I：

10.1007/s10592-009-9819-y

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Ladyfish (Elops saurus Linnaeus) is an economically important marine fish species. 76 microsatellite loci were isolated from an enriched genomic library of Elops saurus. Twelve of these markers were polymorphic in a test population with alleles per locus ranging from three to nine. The number of observed, expected heterozygosity and polymorphism information content (PIC) per locus in 20 individuals ranged from 0.2000 to 1.0000, 0.1897-0.8846, 0.1769-0.8476, respectively. One markers significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of markers. As a result, 12 microsatellite markers probably should provide sufficient level of genetic diversity to investigate the fine-scale population structure, stock management and enhancement, genetic linkage map construction and molecular marker-assisted breeding in Elops saurus Linnaeus.

引用

页码：1799 / 1801

页数：3

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