Genetic analysis of a porcine reproductive and respiratory syndrome virus 1 strain in China with new patterns of amino acid deletions in nsp2, GP3 and GP4

被引：13
|
作者：
Zhang, Qiaoya [1 ,2 ,3 ]
Song, Zhongbao [4 ]
Yu, Ying [1 ,2 ,3 ]
Huang, Juan [1 ,2 ,3 ]
Jiang, Ping [5 ]
Shan, Hu [1 ,2 ,3 ]
机构：
[1] Qingdao Agr Univ, Coll Vet Med, Qingdao 266109, Shandong, Peoples R China
[2] Shandong Prov New Vet Drug Creat Collaborat Innov, Qingdao 266109, Shandong, Peoples R China
[3] Qingdao Vet Biotechnol Engn Res Ctr, Qingdao 266109, Shandong, Peoples R China
[4] Fujian Agr & Forestry Univ, Coll Anim Sci, Key Lab Fujian Taiwan Anim Pathogen Biol, Fuzhou 350002, Fujian, Peoples R China
[5] Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Dis Diagnost & Immunol,Minist Agr, MOE Int Joint Collaborat Res Lab Anim Hlth & Food, Nanjing 210095, Jiangsu, Peoples R China
来源：
MICROBIAL PATHOGENESIS | 2020年 / 149卷
基金：
中国国家自然科学基金;
关键词：
Chinese porcine reproductive and respiratory; syndrome virus 1 (PRRSV 1); Genetic evolution; Amino acid (aa) deletion; Immune cell epitopes; PROTEIN; IDENTIFICATION; REPLICASE; DIVERSITY; EPITOPES; VR-2332; DISEASE; REGIONS;
D O I：
10.1016/j.micpath.2020.104531
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Porcine reproductive and respiratory syndrome virus (PRRSV) 1 and PRRSV 2 have coexisted in China for a very long time. In this study, the complete genomic characterization of a PRRSV 1 strain named KZ2018 was conducted. The results showed that it shared 88.6% identity with Lelystad virus and 81.9-90.8% identities with other Chinese PRRSV 1 strains. Further study showed that its nsp2 protein had a unique discontinuous 6-amino acid (aa) deletion (aa357-360+aa411+aa449). Additionally, its GP3 and GP4 contained a long continuous 18-aa deletion in their overlapped region, which has never been described in other Chinese PRRSV 1 isolates. Amino acid analysis of cell epitopes revealed that GP3(245-256) and GP4(57-68) were the most variable epitopes among different Chinese PRRSV 1 isolates. The results might enrich our knowledge of PRRSV 1 strains in China.
引用
收藏
页数：7
相关论文
共 23 条
  • [1] Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies
    Su, Jia
    Zhou, Lei
    He, Bicheng
    Zhang, Xinhui
    Ge, Xinna
    Han, Jun
    Guo, Xin
    Yang, Hanchun
    VIROLOGICA SINICA, 2019, 34 (06) : 631 - 640
  • [2] Research Progress on the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus
    Lv, Chen
    Yang, Zhiyu
    Lan, Xiaolin
    Liang, Fang
    Kong, Weili
    Wang, Ruining
    Zhao, Mengmeng
    ANIMALS, 2025, 15 (03):
  • [3] Immunogenicity of the envelope GP3 protein of porcine reproductive and respiratory syndrome virus displayed on baculovirus
    Wang, Z. S.
    Xu, X. G.
    Liu, H. J.
    Li, Z. C.
    Ding, L.
    Yu, G. S.
    Tong, D. W.
    ACTA VIROLOGICA, 2011, 55 (02) : 139 - 146
  • [4] Differential host cell gene expression regulated by the porcine reproductive and respiratory syndrome virus GP4 and GP5 glycoproteins
    Lee, CH
    Bachand, A
    Murtaugh, MP
    Yoo, DW
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2004, 102 (03) : 189 - 198
  • [5] GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro
    Costers, Sarah
    Lefebvre, David J.
    Van Doorsselaere, Jan
    Vanhee, Merijn
    Delputte, Peter L.
    Nauwynck, Hans J.
    ARCHIVES OF VIROLOGY, 2010, 155 (03) : 371 - 378
  • [6] A Novel Isolate with Deletion in GP3 Gene of Porcine Reproductive and Respiratory Syndrome Virus from Mid-Eastern China
    Fan, Baochao
    Wang, Hai
    Bai, Juan
    Zhang, Lili
    Jiang, Ping
    BIOMED RESEARCH INTERNATIONAL, 2014, 2014
  • [7] Phages bearing specific peptides with affinity for porcine reproductive and respiratory syndrome virus GP4 protein prevent cell penetration of the virus
    Chen, Wanli
    Cui, Jin
    Wang, Jingyu
    Sun, Yankou
    Ji, Chihai
    Song, Ruyue
    Zeng, Yuchen
    Pan, Haoming
    Sheng, Jinliang
    Zhang, Guihong
    Wang, Heng
    VETERINARY MICROBIOLOGY, 2018, 224 : 43 - 49
  • [8] Generation of Monoclonal Antibody to Porcine Reproductive and Respiratory Syndrome Virus GP4 Protein and Identification of Its Minic Epitopes
    Liu Peng
    Yuan Qing
    Li Wei-qun
    Yin Xue-ting
    Ghulam Abbas
    Li Peng-chong
    Zhang Chao-fan
    Huang Xiao-dan
    Zhang Rui-li
    Li Guang-xing
    Journal of Northeast Agricultural University(English Edition), 2019, 26 (02) : 49 - 59
  • [9] Genetic characterization of a new NSP2-deletion porcine reproductive and Respiratory Syndrome Virus in China
    Xie, Chang-zhan
    Wang, Zheng
    Ha, Zhuo
    Zhang, Ying
    Xie, Yu-biao
    Zhang, He
    Nan, Fu-long
    Zhang, Jin-yong
    Zhao, Guan-yu
    Li, Zhuo-xin
    Li, Cheng-hui
    Yu, Cheng-dong
    Zhang, Ping
    Lu, Hui-jun
    Jin, Ning-yi
    MICROBIAL PATHOGENESIS, 2021, 150
  • [10] Construction and immunological evaluation of recombinant Newcastle disease virus vaccines expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP3/GP5 proteins in pigs
    Zhang, He
    Nan, Fulong
    Li, Zhuoxin
    Zhao, Guanyu
    Xie, Changzhan
    Ha, Zhuo
    Zhang, Jinyong
    Han, Jicheng
    Xiao, Pengpeng
    Zhuang, Xinyu
    Wang, Wei
    Ge, Jinying
    Tian, Mingyao
    Lu, Huijun
    Bu, Zhigao
    Jin, Ningyi
    VETERINARY MICROBIOLOGY, 2019, 239
123