In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration

被引：306

作者：

Huang, Yan ^{[1
,2
]}

Jin, Xiaogang ^{[3
]}

Zhang, Xiaoling ^{[1
,2
]}

Sun, Hongli ^{[1
,2
]}

Tu, Jinwen ^{[1
,2
]}

Tang, Tingting ^{[4
]}

Chang, Jiang ^{[3
]}

Dai, Kerong ^{[1
,2
,5
]}

机构：

[1] Chinese Acad Sci, Key Lab Stem Cell Biol, Inst Hlth Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China

[3] Chinese Acad Sci, State Key Lab High Performance Ceram & Superfine, Shanghai Inst Ceram, Shanghai 200025, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Dept Orthopaed, Peoples Hosp 9, Shanghai 200011, Peoples R China

[5] Minist Educ, Engn Res Ctr Digital Med, Shanghai 200030, Peoples R China

来源：

BIOMATERIALS | 2009年 / 30卷 / 28期

关键词：

Akermanite; beta-Tricalcium phosphate; Extract; Mesenchymal stem cell; Rabbit femur defect models; MARROW STROMAL CELLS; BIOACTIVE GLASS DISSOLUTION; TRICALCIUM PHOSPHATE; CALCIUM PHOSPHATES; HUMAN OSTEOBLASTS; IONIC PRODUCTS; CANCELLOUS BONE; GENE-EXPRESSION; STEM-CELLS; SILICON;

D O I：

10.1016/j.biomaterials.2009.05.077

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This study investigated the effects of a calcium magnesium silicate bioceramic (akermanite) for bone regeneration in vitro and in vivo, with P-tricalcium phosphate (beta-TCP) as a control. In vitro, the human bone marrow-derived mesenchymal stromal cells (hBMSCs) were cultured in an osteogenic medium supplemented with a certain concentration of two bioceramics' extracts for 20 days. An MTT assay showed that akermanite extract promoted proliferation of hBMSC significantly more than did beta-TCP extract. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes such as ALP, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) demonstrated that the osteogenic differentiation of hBMSC was enhanced more by akermanite extract than by beta-TCP extract. In vivo, a histomorphology analysis and histomorphometry of the two porous bioceramics implants in rabbit femur defect models indicated that both in early- and late-stage implantations, akermanite promoted more osteogenesis and biodegradation than did beta-TCP; and in late-stage implantations, the rate of new bone formation was faster in akermanite than in beta-TCP These results suggest that akermanite might be a potential and attractive bioceramic for tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5041 / 5048

页数：8

共 56 条

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