Monoclonal antibody specific for IDH1 R132H mutation

被引：335

作者：

Capper, David ^{[1
]}

Zentgraf, Hanswalter ^{[2
]}

Balss, Joerg ^{[3
]}

Hartmann, Christian ^{[1
,3
]}

von Deimling, Andreas ^{[1
,3
]}

机构：

[1] Heidelberg Univ, Dept Neuropathol, Inst Pathol, D-69120 Heidelberg, Germany

[2] German Canc Res Ctr, Monoclonal Antibody Unit, D-69120 Heidelberg, Germany

[3] German Canc Res Ctr, Clin Cooperat Unit Neuropathol G380, D-69120 Heidelberg, Germany

来源：

ACTA NEUROPATHOLOGICA | 2009年 / 118卷 / 05期

关键词：

IDH1; Monoclonal antibody; Immunohistochemistry; Astrocytoma; Oligodendroglioma; Oligoastrocytoma; CODON; 132; MUTATION; GLIOMAS;

D O I：

10.1007/s00401-009-0595-z

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. We developed a mouse monoclonal antibody targeting the IDH1 R132H mutation. Here, we show the high specificity and sensitivity of this antibody on Western blots and tissue sections from formalin fixed paraffin embedded tumor specimens. This antibody is highly useful for tumor classification, in detecting single infiltrating tumor cells and for the characterization of the cellular role of mutant IDH1 protein.

引用

页码：599 / 601

页数：3

共 50 条

[21] Gaining Insight into the Catalytic Mechanism of the R132H IDH1 Mutant: A Synergistic DFT Cluster and Experimental Investigation
Broome, Joshua A.
Nguyen, Nguyen P.
Baumung, Cassidy R. E.
Chen, Vincent C.
Bushnell, Eric A. C.
BIOCHEMISTRY, 2024, 63 (20) : 2682 - 2691
[22] R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme
Yalaza, Cem
Ak, Handan
Cagli, Mehmet Sedat
Ozgiray, Erkin
Atay, Sevcan
Aydin, Hikmet Hakan
ANNALS OF CLINICAL AND LABORATORY SCIENCE, 2017, 47 (03) : 362 - 364
[23] Characterization of IDH1 p.R132H Mutant Clones Using Mutation-specific Antibody in Myeloid Neoplasms
Kurt, Habibe
Bueso-Ramos, Carlos E.
Khoury, Joseph D.
Routbort, Mark J.
Kanagal-Shamanna, Rashmi
Patel, Umang V.
Jorgensen, Jeffrey L.
Wang, Sa A.
Ravandi, Farhad
DiNardo, Courtney
Luthra, Rajyalakshmi
Medeiros, L. Jeffrey
Patel, Keyur P.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 2018, 42 (05) : 569 - 577
[24] A Case of Ollier's Disease with P53 Mutation Positive and IDH1 (R132H) Negative Multicentric Gliomas
Nishtha, Y.
Maya, B.
Shetty, Safal S.
Ganaraj, V. H.
Nupur, P.
Yasha, T. C.
Netravathi, M.
NEUROLOGY INDIA, 2020, 68 (03) : 657 - 659
[25] C-terminally truncated form of αB-crystallin is associated with IDH1 R132H mutation in anaplastic astrocytoma
Nuraly K. Avliyakulov
Kavitha S. Rajavel
Khanh Minh T. Le
Lea Guo
Leili Mirsadraei
William H. Yong
Linda M. Liau
Sichen Li
Albert Lai
Phioanh L. Nghiemphu
Timothy F. Cloughesy
Michael Linetsky
Michael J. Haykinson
Whitney B. Pope
Journal of Neuro-Oncology, 2014, 117 : 53 - 65
[26] A novel monoclonal antibody GMab-m1 specifically recognizes IDH1-R132G mutation
Kato, Yukinari
Natsume, Atsushi
Kaneko, Mika Kato
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 432 (04) : 564 - 567
[27] The prognostic IDH1 R132 mutation is associated with reduced NADP+-dependent IDH activity in glioblastoma
Bleeker, Fonnet E.
Atai, Nadia A.
Lamba, Simona
Jonker, Ard
Rijkeboer, Denise
Bosch, Klazien S.
Tigchelaar, Wikky
Troost, Dirk
Vandertop, W. Peter
Bardelli, Alberto
Van Noorden, Cornelis J. F.
ACTA NEUROPATHOLOGICA, 2010, 119 (04) : 487 - 494
[28] Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma
Shayanfar, Nasrin
Zare-Mirzaie, Ali
Mohammadpour, Mahsa
Jafari, Ensieh
Mehrtash, Amirhosein
Emtiazi, Nikoo
Tajik, Fatemeh
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2023, 149 (08) : 4253 - 4267
[29] Segregation of Non-p.R132H Mutations in IDH1 in Distinct Molecular Subtypes of Glioma
Gravendeel, Lonneke A. M.
Kloosterhof, Nanne K.
Bralten, Linda B. C.
van Marion, Ronald
Dubbink, Hendrikus Jan
Dinjens, Winand
Bleeker, Fonnet E.
Hoogenraad, Casper C.
Michiels, Erna
Kros, Johan M.
van den Bent, Martin
Smitt, Peter A. E. Sillevis
French, Pim J.
HUMAN MUTATION, 2010, 31 (03) : E1186 - E1199
[30] Frequent false-negative immunohistochemical staining with IDH1 (R132H)-specific H09 antibody on frozen section control slides: a potential pitfall in glioma diagnosis
Yoshida, Akihiko
Satomi, Kaishi
Ohno, Makoto
Matsushita, Yuko
Takahashi, Masamichi
Miyakita, Yasuji
Hiraoka, Nobuyoshi
Narita, Yoshitaka
Ichimura, Koichi
HISTOPATHOLOGY, 2019, 74 (02) : 350 - 354

← 1 2 3 4 5 →