An integrated platform for a high-throughput pharmacokinetic study of glycosides using a boronic acid-functionalized 96-well glass plate

被引：2
|
作者：
Zhao, Ningning [1 ,2 ,3 ]
Gu, Qianqian [1 ,2 ,3 ]
Liu, Zhiqiang [1 ,2 ,3 ,4 ]
Song, Fengrui [1 ,2 ,3 ,4 ]
Pi, Zifeng [1 ,2 ]
Liu, Shu [1 ,2 ]
机构：
[1] Chinese Acad Sci, Natl Ctr Mass Spectrometry Changchun, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Jilin Prov Key Lab Chinese Med Chem & Mass Spectr, Changchun 130022, Jilin, Peoples R China
[3] Univ Sci & Technol China, Hefei 230029, Anhui, Peoples R China
[4] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Elect Chem, Changchun 130022, Jilin, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 64期
基金：
中国国家自然科学基金;
关键词：
MAGNETIC NANOPARTICLES; RECOGNITION; AFFINITY;
D O I：
10.1039/c9cc04045e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The novel Vial@FPBA strategy was established for a large-scale pharmacokinetic study of glycosides, during which glycosides were absorbed into a boronic acid-functionalized 96-well glass plate and directly desorbed for UHPLC-MS/MS analysis. Hence, specific and high-throughput glycoside enrichment was achieved simultaneously. The LODs were reduced up to 50 times compared to the case of the methanol method. Meanwhile, sample pre-processing time was greatly saved by skipping the protein sedimentation and supernatant concentration steps.
引用
收藏
页码：9543 / 9546
页数：4
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