Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future

被引:67
作者
Gosalvez, Jaime [1 ]
Tvrda, Eva [2 ]
Agarwal, Ashok [3 ]
机构
[1] Univ Autonoma Madrid, Dept Biol, Edificio Biol,Darwin 2, E-28049 Madrid, Spain
[2] Slovak Univ Agr, Dept Anim Physiol, Tr A Hlinku 2, Nitra 94976, Slovakia
[3] Cleveland Clin, Amer Ctr Reprod Med, Mail Code X-11,10681 Carnegie Ave, Cleveland, OH 44195 USA
关键词
Antioxidants; Male infertility; Oxidative stress; Reactive oxygen species; Semen; Spermatozoa; Superoxide; OXYGEN SPECIES PRODUCTION; NITROBLUE TETRAZOLIUM REDUCTION; TOTAL ANTIOXIDANT CAPACITY; OXIDATIVE STRESS; HUMAN-SPERM; HUMAN SPERMATOZOA; MALE-INFERTILITY; COLORIMETRIC ASSAY; SEMINAL LEUKOCYTES; LIPID-PEROXIDATION;
D O I
10.1007/s10815-017-0912-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Oxidative stress is a well-established cause of male infertility, with reactive oxygen species (ROS) impairing sperm production, motility, membrane, and DNA integrity. Currently, most clinics do not test infertile patients for the imbalance between ROS generation and the ability of the antioxidants to scavenge them, although there is a clear need for andrology laboratories to be able to identify and/or quantify seminal oxidative stress. As such there is a clinical urgency for an inexpensive and easy-to-perform assay able to identify oxidative stress in semen. The aim of this review is to provide information on the currently available methods to assess and quantify ROS and particularly superoxide in male reproductive cells, tissues, and fluids which may have a significant clinical utility in identifying men with impaired fertility associated with oxidative stress. Through a deeper understanding of oxidative stress and its assessment options, clinical andrology labs may better assist patients to achieve increased rates of fertility and pregnancy.
引用
收藏
页码:697 / 707
页数:11
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