Asymmetric Distribution of Cholesterol in Unilamellar Vesicles of Monounsaturated Phospholipids

被引：26

作者：

Kucerka, Norbert ^{[1
,2
]}

Nieh, Mu-Ping ^{[1
]}

Katsaras, John ^{[1
,3
,4
,5
]}

机构：

[1] CNR, Canadian Neutron Beam Ctr, Chalk River, ON K0J 1J0, Canada

[2] Comenius Univ, Fac Pharm, Dept Phys Chem Drugs, Bratislava 83232, Slovakia

[3] Brock Univ, Dept Phys, St Catharines, ON L2S 3A1, Canada

[4] Univ Guelph, Guelph Waterloo Phys Inst, Guelph, ON N1G 2W1, Canada

[5] Univ Guelph, Biophys Interdepartmental Grp, Guelph, ON N1G 2W1, Canada

来源：

LANGMUIR | 2009年 / 25卷 / 23期

基金：

美国国家科学基金会;

关键词：

X-RAY-SCATTERING; PHASE-TRANSITION; MEMBRANE CURVATURE; MAXIMUM SOLUBILITY; LIPID-BILAYERS; MODEL; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLCHOLINE; COOPERATIVITY; SEPARATION;

D O I：

10.1021/la9020299

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have studied the effect of cholesterol oil curved bilayers using 600 angstrom unilamellar vesicles made of monounsaturated lipids. From small-angle X-ray scattering experiments we were able to detect an asymmetric distribution of lipid densities across certain bilayers. We discovered that, with the exception of diC24 : 1PC bilayers, monounsaturated diacylphosphatidylcholine lipids (diCn : 1PC, n = 14, 16, 18, 20, and 22) form symmetric bilayers. However, the addition of 44 mol % cholesterol resulted in some of these bilayers (i.e., n = 14, 16, and 18) to become asymmetric, where cholesterol was found to distribute unequally between the bilayer's two leaflets. This finding is potentially of relevance to biological membranes made up of different types of lipids and whose local curvature may be dictated by lipid composition.

引用

页码：13522 / 13527

页数：6

共 30 条

[1] Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes [J].