Pupil filters for extending the field-of-view in light-sheet microscopy

被引：19
|
作者：
Wilding, Dean [1 ]
Pozzi, Paolo [1 ]
Soloviev, Oleg [1 ,3 ]
Vdovin, Gleb [1 ,3 ]
Sheppard, Colin J. [2 ]
Verhaegen, Michel [1 ]
机构：
[1] Delft Univ Technol, Delft Ctr Syst & Control, Mekelweg 2, NL-2628 CD Delft, Netherlands
[2] Italian Inst Technol, Via Morego 30, I-16163 Genoa, Italy
[3] Flexible Opt BV, Polakweg 10-11, NL-2288 GG Rijswijk, Netherlands
来源：
OPTICS LETTERS | 2016年 / 41卷 / 06期
基金：
欧洲研究理事会;
关键词：
PLANE ILLUMINATION; BEAM;
D O I：
10.1364/OL.41.001205
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Pupil filters, represented by binary phase modulation, have been applied to extend the field of view of a light-sheet fluorescence microscope. Optimization has been used, first numerically to calculate the optimum filter structure and then experimentally, to scale and align the numerically synthesized filter in the microscope. A significant practical extension of the field of view has been observed, making the reported approach a valuable tool on the path to wide-field light-sheet microscopy. (C) 2016 Optical Society of America
引用
收藏
页码：1205 / 1208
页数：4
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