Pupil filters for extending the field-of-view in light-sheet microscopy

被引：19

作者：

Wilding, Dean ^{[1
]}

Pozzi, Paolo ^{[1
]}

Soloviev, Oleg ^{[1
,3
]}

Vdovin, Gleb ^{[1
,3
]}

Sheppard, Colin J. ^{[2
]}

Verhaegen, Michel ^{[1
]}

机构：

[1] Delft Univ Technol, Delft Ctr Syst & Control, Mekelweg 2, NL-2628 CD Delft, Netherlands

[2] Italian Inst Technol, Via Morego 30, I-16163 Genoa, Italy

[3] Flexible Opt BV, Polakweg 10-11, NL-2288 GG Rijswijk, Netherlands

来源：

OPTICS LETTERS | 2016年 / 41卷 / 06期

基金：

欧洲研究理事会;

关键词：

PLANE ILLUMINATION; BEAM;

D O I：

10.1364/OL.41.001205

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Pupil filters, represented by binary phase modulation, have been applied to extend the field of view of a light-sheet fluorescence microscope. Optimization has been used, first numerically to calculate the optimum filter structure and then experimentally, to scale and align the numerically synthesized filter in the microscope. A significant practical extension of the field of view has been observed, making the reported approach a valuable tool on the path to wide-field light-sheet microscopy. (C) 2016 Optical Society of America

引用

页码：1205 / 1208

页数：4

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