Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag

被引：27

作者：

Qiu, Jian-Tai

Chang, Ting- Chang

Lin, Cheng-Tao

Chen, Yi-Ming

Li, Frank Q.

Soong, Yung-Kuei

Lai, Chyong-Huey

机构：

[1] Chang Gung Mem Hosp, Dept Obstet & Gynecol, Tao Yuan 333, Taiwan

[2] Chang Gung Univ, Coll Med, Dept Life Sci, Tao Yuan 333, Taiwan

[3] Natl Yang Ming Univ, AIDs Prevent & Res Ctr, Taipei 112, Taiwan

[4] Vaxim Inc, Rockville, MD 20850 USA

来源：

VACCINE | 2007年 / 25卷 / 02期

关键词：

GM-CSF; DNA vaccine; HIV-1; T-lymphocytes; immunity; COLONY-STIMULATING FACTOR; T-CELL RESPONSES; VIRUS TYPE-1 GAG; DENDRITIC CELLS; PLASMID DNA; FUSION PROTEIN; PHASE-I; EXPRESSION; IMMUNOGENICITY; ANTIGEN;

D O I：

10.1016/j.vaccine.2006.07.034

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immunomodulatory cytokine. Here we generated a novel codon-optimized murine GM-CSF gene as an adjuvant. The codon-optimized GM-CSF gene significantly increased protein expression levels in all cells tested. Although injection of the wild-type GM-CSF plasmids adjuvanted HIV-1 Gag DNA vaccine induced detectable immune responses. co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the DNA vaccine resulted in a strong antibody and CTL responses and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag. This novel codon-optimized GM-CSF gene offers a practical molecular strategy for potentiating immune responses to vaccines as well as other immunotherapeutic strategies. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：253 / 263

页数：11