Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging

被引：3
|
作者：
Bub, Gil [1 ]
Tecza, Matthias [2 ]
Helmes, Michiel [1 ]
Lee, Peter [1 ]
Kohl, Peter [1 ]
机构：
[1] Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 2JD, England
[2] Univ Oxford, Dept Phys, Oxford OX1 2JD, England
来源：
NATURE METHODS | 2010年 / 7卷 / 03期
基金：
英国生物技术与生命科学研究理事会;
关键词：
SIGNALS; RAT;
D O I：
10.1038/NMETH.1429
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.
引用
收藏
页码：209 / U66
页数：5
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