Adaptive Optics in Three-Photon Fluorescence Microscopy

被引:0
|
作者
Sinefeld, David [1 ]
Paudel, Hari P. [2 ]
Ouzounov, Dimitre G. [1 ]
Bifano, Thomas G. [2 ]
Xu, Chris [1 ]
机构
[1] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA
[2] Boston Univ, Photon Ctr, Boston, MA 02215 USA
关键词
SCATTERING MEDIA;
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
We demonstrate three-photon fluorescence adaptive-optics system based on 1660-nm femtosecond pulses and MEMS spatial light modulator. We use the higher nonlinearity of the signal resulting in x700 improvement in fluorescence beads signal after aberrations correction.
引用
收藏
页数:2
相关论文
共 50 条
  • [31] Enhanced Melanin Fluorescence by Stepwise Three-photon Excitation
    Kerimo, Josef
    Rajadhyaksha, Milind
    DiMarzio, Charles A.
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2011, 87 (05) : 1042 - 1049
  • [32] Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging
    Pranoy Sahu
    Nirmal Mazumder
    Lasers in Medical Science, 2020, 35 : 317 - 328
  • [33] A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging
    Tao, Xiaodong
    Lu, Ju
    Lam, Tuwin
    Rodriguez, Ramiro
    Zuo, Yi
    Kubby, Joel
    NEURAL IMAGING AND SENSING, 2017, 10051
  • [34] Fluorescence digital holographic adaptive optics microscopy
    Man, Tianlong
    Wan, Yuhong
    Wang, Dayong
    HOLOGRAPHY: ADVANCES AND MODERN TRENDS IV, 2015, 9508
  • [35] Intravital three-photon microscopy of entire mouse lymph node
    Choe, Kibaek
    Hontani, Yusaku
    Wang, Tianyu
    Ouzounov, Dimitre
    Lai, Kristine
    Signh, Ankur
    Beguelin, Wendy
    Melnick, Ari M.
    Xu, Chris
    JOURNAL OF IMMUNOLOGY, 2020, 204 (01):
  • [36] Simultaneous third-harmonic generation and three-photon microscopy
    Fittinghoff, David N.
    Squier, Jeffrey A.
    Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest, 2000,
  • [37] Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy
    Kim, Hyeonggeon
    Natan, Ryan
    Chen, Wei
    Winans, Amy m.
    Fan, Jiang lan
    Isacoff, Ehud
    Ji, Na
    OPTICS EXPRESS, 2025, 33 (01): : 680 - 693
  • [38] Advances in adaptive optics-based two-photon fluorescence microscopy for brain imaging
    Sahu, Pranoy
    Mazumder, Nirmal
    LASERS IN MEDICAL SCIENCE, 2020, 35 (02) : 317 - 328
  • [39] Optofluidic adaptive optics in multi-photon microscopy
    Sohmen, Maximilian
    Munoz-Bolanos, Juan D.
    Rajaeipour, Pouya
    Ritsch-Marte, Monika
    Ataman, Caglar
    Jesacher, Alexander
    BIOMEDICAL OPTICS EXPRESS, 2023, 14 (04) : 1562 - 1578
  • [40] Rapid volumetric imaging with Bessel-Beam three-photon microscopy
    Chen, Bingying
    Huang, Xiaoshuai
    Gou, Dongzhou
    Zeng, Jianzhi
    Chen, Guoqing
    Pang, Meijun
    Hu, Yanhui
    Zhao, Zhe
    Zhang, Yunfeng
    Zhou, Zhuan
    Wu, Haitao
    Cheng, Heping
    Zhang, Zhigang
    Xu, Chris
    Li, Yulong
    Chen, Liangyi
    Wang, Aimin
    BIOMEDICAL OPTICS EXPRESS, 2018, 9 (04): : 1992 - 2000