Plant regeneration via organogenesis or somatic embryogenesis in sugarcane: Histological studies

Embryogenic callus was obtained by culturing young leaves of sugarcane on modified MS medium containina 4mg/l 2,4-D. Many embryos were formed when the callus was transferred on the medium containing low concentration of 2,4-D (0.5 mg/l). The callus transferred on the medium without 2,4-D turned black after 7 to 10 days of sub-culturing. Embryos were cytoplasmically rich and showed the typical organization of grass embryos. Somatic embryos were regenerated on the regeneration media (MS salts + 2 mg/l each Kin, IAA and IBA + 2% sugar) and profused rooting was observed on medium containing MS + 1 mg/l IBA + 6% sugar. Rooted plantlets were transferred into the field after hardening for further evaluation.