Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation

被引：13
|
作者：
Lovrinovic, Marina [1 ]
Fruk, Ljiljana [1 ]
Schroeder, Hendrik [1 ]
Niemeyer, Christof M. [1 ]
机构：
[1] Univ Dortmund, Fachbereich Chem, D-44227 Dortmund, Germany
来源：
CHEMICAL COMMUNICATIONS | 2007年 / 04期
关键词：
D O I：
10.1039/b614978b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry.
引用
收藏
页码：353 / 355
页数：3
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