Bio-inspired chiral self-assemblies promoted neuronal differentiation of retinal progenitor cells through activation of metabolic pathway

被引：28

作者：

Sun, Na ^{[1
]}

Dou, Xiaoqiu ^{[2
]}

Tang, Zhimin ^{[1
]}

Zhang, Dandan ^{[1
]}

Ni, Ni ^{[1
]}

Wang, Jiajing ^{[1
]}

Gao, Huiqin ^{[1
]}

Ju, Yahan ^{[1
]}

Dai, Xiaochan ^{[1
]}

Zhao, Changli ^{[2
]}

Gu, Ping ^{[1
]}

Ji, Jing ^{[1
]}

Feng, Chuanliang ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Peoples Hosp 9, Dept Ophthalmol, Shanghai Key Lab Orbital Dis & Ocular Oncol,Sch M, Shanghai 200011, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Mat Sci & Engn, State Key Lab Met Matrix Composites, Dongchuan Rd 800, Shanghai 200240, Peoples R China

来源：

BIOACTIVE MATERIALS | 2021年 / 6卷 / 04期

基金：

国家重点研发计划;

关键词：

Chiral structures; Self-assembly; Nanofibers; Retinal progenitor cell; Neuronal differentiation; PROLIFERATION; CHEMISTRY; MATRIX; ACID;

D O I：

10.1016/j.bioactmat.2020.09.027

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Retinal degeneration is a main class of ocular diseases. So far, retinal progenitor cell (RPC) transplantation has been the most potential therapy for it, in which promoting RPCs neuronal differentiation remains an unmet challenge. To address this issue, innovatively designed (L/D)-phenylalanine based chiral nanofibers (LPG and DPG) are employed and it finds that chirality of fibers can efficiently regulate RPCs differentiation. qPCR, western blot, and immunofluorescence analysis show that right-handed helical DPG nanofibers significantly promote RPCs neuronal differentiation, whereas left-handed LPG nanofibers decrease this effect. These effects are mainly ascribed to the stereoselective interaction between chiral helical nanofibers and retinol-binding protein 4 (RBP4, a key protein in the retinoic acid (RA) metabolic pathway). The findings of chirality-dependent neuronal differentiation provide new strategies for treatment of neumdegenerative diseases via optimizing differentiation of transplanted stem cells on chiral nanofibers.

引用

页码：990 / 997

页数：8

共 32 条

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