F-16438s, novel binding inhibitors of CD44 and hyaluronic acid - I. Establishment of an assay method and biological activity

In an attempt to obtain inhibitors of hyaluronic acid (HA) binding to its receptor, CD44, we established an efficient assay method to detect and quantify binding using fluorescein-labeled HA and HEK293 cells stably expressing CD44. As a result of the screening of culture broths of microorganisms, we found fungus strain Gloeoporus dichrous SANK 30502 produced inhibitory activity in this new assay. Five compounds, F-16438 A, B, E, F and G, were isolated from the fermentation broths, and their IC50 values were determined to be 10.3, 13.5, 27.3, 12.0 and 13.0 mu M, respectively F-16438 A, B, E, F and G are the first reported inhibitors of binding HA to CD44. F-1643 8 A, B, E and F have novel structures.