Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

被引：12

作者：

Liu, Chen ^{[1
,2
,3
]}

Malek, Michael ^{[2
]}

Poon, Ivan ^{[4
]}

Jiang, Lanzhou ^{[4
]}

Siddiquee, Arif M. ^{[2
,5
]}

Sheppard, Colin J. R. ^{[6
]}

Roberts, Ann ^{[7
]}

Quiney, Harry ^{[7
]}

Zhang, Douguo ^{[3
]}

Yuan, Xiaocong ^{[1
]}

Lin, Jiao ^{[1
,7
,8
]}

Depeursinge, Christian ^{[9
,10
]}

Marquet, Pierre ^{[11
,12
,13
,14
]}

Kou, Shan Shan ^{[2
,4
,6
,9
]}

机构：

[1] Shenzhen Univ, Nanophoton Res Ctr, Shenzhen Key Lab Microscale Opt Informat Technol, Shenzhen 518060, Peoples R China

[2] La Trobe Univ, La Trobe Inst Mol Sci LIMS, Dept Chem & Phys, Melbourne, Vic 3086, Australia

[3] Univ Sci & Technol China, Dept Opt & Opt Engn, Anhui Key Lab Optoelect Sci & Technol, Hefei 230026, Anhui, Peoples R China

[4] La Trobe Univ, La Trobe Inst Mol Sci LIMS, Dept Biochem & Genet, Melbourne, Vic 3086, Australia

[5] ARC, Ctr Excellence Adv Mol Imaging, Canberra, ACT, Australia

[6] Ist Italiano Tecnol, I-16163 Genoa, Italy

[7] Univ Melbourne, Sch Phys, Melbourne, Vic 3010, Australia

[8] RMIT Univ, Sch Engn, Melbourne, Vic 3001, Australia

[9] Ecole Polytech Fed Lausanne, Microvis & Microdiagnost Grp SCI STI CHD, CH-1015 Lausanne, Switzerland

[10] KAUST, Biol & Environm Sci & Engn Div, Lab Cellular Imaging & Energet, Thuwal 239556900, Saudi Arabia

[11] CHU Vaudois, Dept Psychiat, Joint Int Res Unit Neurodev & Child Psychiat, Lausanne, Switzerland

[12] Univ Laval, Quebec City, PQ, Canada

[13] Inst Univ Sante Mentale Quebec, Quebec City, PQ, Canada

[14] Univ Laval, Dept Psychiat & Neurosci, Ctr Opt Photon & Laser, 2375 Rue Terrasse, Quebec City, PQ G1V 0A6, Canada

来源：

PHOTONICS RESEARCH | 2019年 / 7卷 / 09期

基金：

澳大利亚研究理事会; 中国国家自然科学基金;

关键词：

ELECTRON-MICROSCOPY; PHASE; RECONSTRUCTION; FIELD;

D O I：

10.1364/PRJ.7.001042

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model. (C) 2019 Chinese Laser Press.

引用

页码：1042 / 1050

页数：9

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