Silver staining (Campbell-Switzer) of neuronal α-synuclein assemblies induced by multiple system atrophy and Parkinson's disease brain extracts in transgenic mice

被引：32
作者：
Lavenir, Isabelle [1 ]
Passarella, Daniela [1 ]
Masuda-Suzukake, Masami [1 ]
Curry, Annabelle [1 ]
Holton, Janice L. [2 ]
Ghetti, Bernardino [3 ]
Goedert, Michel [1 ]
机构：
[1] MRC Lab Mol Biol, Cambridge CB2 0QH, England
[2] UCL, Queen Sq Inst Neurol, London WC1N 1PJ, England
[3] Indiana Univ Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA
来源：
ACTA NEUROPATHOLOGICA COMMUNICATIONS | 2019年 / 7卷 / 01期
基金：
英国医学研究理事会;
关键词：
alpha-Synuclein; Multiple system atrophy; Parkinson's disease; Seeding; Silver staining; Transgenic; GLIAL CYTOPLASMIC INCLUSIONS; MESSENGER-RNA EXPRESSION; LEWY BODIES; CRYO-EM; TRANSMISSION; OLIGODENDROCYTES; DEMENTIA; PHOSPHORYLATION; DEGENERATION; FILAMENTS;
D O I：
10.1186/s40478-019-0804-5
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Synucleinopathies [Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA)] share filamentous alpha-synuclein assemblies in nerve cells and glial cells. We compared the abilities of brain extracts from MSA and PD patients to induce neuronal alpha-synuclein assembly and neurodegeneration following intracerebral injection in heterozygous mice transgenic for human mutant A53T alpha-synuclein. MSA extracts were more potent than PD extracts in inducing alpha-synuclein assembly and in causing neurodegeneration. MSA assemblies were Campbell-Switzer- and Gallyas-silver-positive, whereas PD assemblies were only Campbell-Switzer-positive, in confirmation of previous findings. However, induced alpha-synuclein inclusions were invariably Campbell-Switzer-positive and Gallyas-negative, irrespective of whether MSA or PD brain extracts were injected. The alpha-synuclein inclusions of non-injected homozygous mice transgenic for A53T alpha-synuclein were also Campbell-Switzer-positive and Gallyas-negative. These findings demonstrate that transgene expression and its intracellular environment dominated over the silver staining properties of the conformers of assembled alpha-synuclein.
引用
收藏
页数：11
相关论文
共 54 条
[1]   Phosphorylation of Ser-129 is the dominant pathological modification of α-synuclein in familial and sporadic Lewy body disease [J].
Anderson, John P. ;
Walker, Donald E. ;
Goldstein, Jason M. ;
de laat, Rian ;
Banducci, Kelly ;
Caccavello, Russell J. ;
Barbour, Robin ;
Huang, Jiping ;
Kling, Kristin ;
Lee, Michael ;
Diep, Linnea ;
Keim, Pamela S. ;
Shen, Xiaofeng ;
Chataway, Tim ;
Schlossmacher, Michael G. ;
Seubert, Peter ;
Schenk, Dale ;
Sinha, Sukanto ;
Gai, Wei Ping ;
Chilcote, Tamie J. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (40) :29739-29752
[2]  
[Anonymous], 2019, ELIFE, DOI DOI 10.7554/eLife.43584
[3]   Alpha-synuclein mRNA expression in oligodendrocytes in MSA [J].
Asi, Yasmine T. ;
Simpson, Julie E. ;
Heath, Paul R. ;
Wharton, Stephen B. ;
Lees, Andrew J. ;
Revesz, Tamas ;
Houlden, Henry ;
Holton, Janice L. .
GLIA, 2014, 62 (06) :964-970
[4]   Structural and functional characterization of two alpha-synuclein strains [J].
Bousset, Luc ;
Pieri, Laura ;
Ruiz-Arlandis, Gemma ;
Gath, Julia ;
Jensen, Poul Henning ;
Habenstein, Birgit ;
Madiona, Karine ;
Olieric, Vincent ;
Boeckmann, Anja ;
Meier, Beat H. ;
Melki, Ronald .
NATURE COMMUNICATIONS, 2013, 4
[5]   Silver staining method for demonstrating Lewy bodies in Parkinson's disease and argyrophilic oligodendrocytes in multiple system atrophy [J].
Braak, E ;
Braak, H .
JOURNAL OF NEUROSCIENCE METHODS, 1999, 87 (01) :111-115
[6]  
Campbell S., 1987, Soc Neurosci Abstr, V13, P67
[7]   Transmission and spreading of tauopathy in transgenic mouse brain [J].
Clavaguera, Florence ;
Bolmont, Tristan ;
Crowther, R. Anthony ;
Abramowski, Dorothee ;
Frank, Stephan ;
Probst, Alphonse ;
Fraser, Graham ;
Stalder, Anna K. ;
Beibel, Martin ;
Staufenbiel, Matthias ;
Jucker, Mathias ;
Goedert, Michel ;
Tolnay, Markus .
NATURE CELL BIOLOGY, 2009, 11 (07) :909-U325
[8]   Characterisation of isolated α-synuclein filaments from substantia nigra of Parkinson's disease brain [J].
Crowther, RA ;
Daniel, SE ;
Goedert, M .
NEUROSCIENCE LETTERS, 2000, 292 (02) :128-130
[9]   Expanding the spectrum of neuronal pathology in multiple system atrophy [J].
Cykowski, Matthew D. ;
Coon, Elizabeth A. ;
Powell, Suzanne Z. ;
Jenkins, Sarah M. ;
Benarroch, Eduardo E. ;
Low, Phillip A. ;
Schmeichel, Ann M. ;
Parisi, Joseph E. .
BRAIN, 2015, 138 :2293-2309
[10]   Comparative analyses of the in vivo induction and transmission of -synuclein pathology in transgenic mice by MSA brain lysate and recombinant -synuclein fibrils [J].
Dhillon, Jess-Karan S. ;
Trejo-Lopez, Jorge A. ;
Riffe, Cara ;
Levites, Yona ;
Sacino, Amanda N. ;
Borchelt, David R. ;
Yachnis, Anthony Y. ;
Giasson, Benoit I. .
ACTA NEUROPATHOLOGICA COMMUNICATIONS, 2019, 7 (1) :80
123456