Nanobodies as Probes for Protein Dynamics in Vitro and in Cells

被引：83

作者：

Dmitriev, Oleg Y. ^{[1
]}

Lutsenko, Svetlana ^{[2
]}

Muyldermans, Serge ^{[3
]}

机构：

[1] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

[2] Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USA

[3] Vrije Univ Brussel, Unit Cellular & Mol Immunol, B-1050 Brussels, Belgium

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2016年 / 291卷 / 08期

基金：

加拿大健康研究院; 美国国家卫生研究院;

关键词：

nuclear magnetic resonance (NMR); protein domain; protein dynamic; single-domain antibody (sdAb; nanobody); X-ray crystallography; nanobody; protein dynamics; protein structure; protein engineering; BETA(2)-ADRENERGIC RECEPTOR FUNCTION; SINGLE-DOMAIN ANTIBODIES; WILSONS-DISEASE PROTEIN; HEAVY-CHAIN ANTIBODIES; X-RAY-STRUCTURE; CRYSTAL-STRUCTURE; STRUCTURAL INSIGHTS; BINDING-PROTEINS; MULTIDRUG TRANSPORTER; COUPLED RECEPTOR;

D O I：

10.1074/jbc.R115.679811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanobodies are the recombinant antigen-recognizing domains of the minimalistic heavy chain-only antibodies produced by camels and llamas. Nanobodies can be easily generated, effectively optimized, and variously derivatized with standard molecular biology protocols. These properties have triggered the recent explosion in the nanobody use in basic and clinical research. This review focuses on the emerging use of nanobodies for understanding and monitoring protein dynamics on the scales ranging from isolated protein domains to live cells, from nanoseconds to hours. The small size and high solubility make nanobodies uniquely suited for studying protein dynamics by NMR. The ability to produce conformation-sensitive nanobodies in cells enables studies that link structural dynamics of a target protein to its cellular behavior. The link between in vitro and in-cell dynamics, afforded by nanobodies, brings the analysis of such important events as receptor signaling, membrane protein trafficking, and protein interactions to the next level of resolution.

引用

页码：3767 / 3775

页数：9

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