Production of an acidic and thermostable lipase of the mesophilic fungus Penicillium simplicissimum by solid-state fermentation

被引：121

作者：

Gutarra, Melissa L. E.

Godoy, Mateus G.

Maugeri, Francisco ^{[2
]}

Rodrigues, Maria Isabel ^{[2
]}

Freire, Denise M. G. ^{[1
]}

Castilho, Leda R. ^{[3
]}

机构：

[1] Univ Fed Rio de Janeiro, Ctr Tecnol 149, Inst Quim, Dept Bioquim,Lab Biotecnol Microbiana 549 1, BR-21941909 Rio De Janeiro, Brazil

[2] Univ Estadual Campinas UNICAMP, FEA, Dept Engn Alimentos, Campinas, SP, Brazil

[3] Univ Fed Rio de Janeiro, COPPE, Programa Engn Quim, BR-21941909 Rio De Janeiro, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 21期

关键词：

Solid-state fermentation; Babassu cake; Penicillium simplicissimum; Acidic lipase; Thermal stability; PURIFICATION; OIL; RESTRICTUM; ENZYMES; ADVANTAGES; CAKE;

D O I：

10.1016/j.biortech.2008.08.050

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The production of a lipase by a wild-type Brazilian strain of Penicillium simplicissimium in solid-state fermentation of babassu cake, an abundant residue of the oil industry, was studied. The enzyme production reached about 90 U/g in 72 h, with a specific activity of 4.5 U/mg of total proteins. The Crude lipase showed high activities at 35-60 degrees C and pH 4.0-6.0, with a maximum activity at 50 degrees C and pH 4.0-5.0. Enzyme stability was enhanced at pH 5.0 and 6.0, with a maximum half-life of 5.02 h at 50 degrees C and pH 5.0. Thus, this lipase shows a thermophilic and thermostable behavior, what is not common among lipases from mesophilic filamentous fungi. The crude enzyme catalysed the hydrolysis of triglycerides and p-nitrophenyl esters (C4:0-C18:0), preferably acting on substrates with medium-chain fatty acids. This non-purified lipase in addition to interesting properties showed a reduced production cost making feasible its applicability in many fields. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5249 / 5254

页数：6

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