Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

被引：35

作者：

Liu, Weimiao ^{[1
,2
]}

Xu, Liai ^{[1
,2
]}

Lin, Hui ^{[3
]}

Cao, Jiashu ^{[1
,2
,4
]}

机构：

[1] Zhejiang Univ, Inst Vegetable Sci, Lab Cell & Mol Biol, Hangzhou 310058, Peoples R China

[2] Minist Agr, Key Lab Hort Plant Growth Dev & Qual Improvement, Hangzhou 310058, Peoples R China

[3] Fujian Acad Agr Sci, Crop Res Inst, Fuzhou 350013, Peoples R China

[4] Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Peoples R China

来源：

GENES | 2021年 / 12卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Arabidopsis thaliana; expansin genes; AtEXPA4; AtEXPB5; pollen tube growth; root elongation; CELL-WALL INTEGRITY; PSI BETA-BARREL; PECTIN METHYLESTERASE; POLYGALACTURONASE GENE; BRASSICA-CAMPESTRIS; SYSTEM ARCHITECTURE; ANTHER DEVELOPMENT; EXPRESSION; EXTENSINS; PROMOTER;

D O I：

10.3390/genes12020249

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4 atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.

引用

页码：1 / 16

页数：16