Cloning and overexpression of a Paenibacillus β-glucanase in Pichia pastoris:: Purification and characterization of the recombinant enzyme

Isolation, expression, and characterization of a novel endo-beta-1,3(4)-D-glucanase with high specific activity and homology to Bacillus lichenases is described. One clone was screened from a genomic library of Paenibacillus sp. F-40, using lichenan-containing plates. The nucleotide sequence of the clone contains an ORF consisting of 717 nucleotides, encoding a beta-glucanase protein of 238 amino acids and 26 residues of a putative signal peptide at its N-terminus. The amino acid sequence showed the highest similarity of 87% to other beta-1,3-1,4-glucanases of Bacillus. The gene fragment Bg1 containing the mature glucanase protein was expressed in Pichia pastoris at high expression level in a 34 high-cell-density fermenter. The purified recombinant enzyme Bg1 showed activity against barley beta-glucan, lichenan, and laminarin. The gene encodes an endo-beta-1,3(4)-D-glucanase (E.C.3.2.1.6). When lichenan was used as substrate, the optimal pH was 6.5, and the optimal temperature was 60 degrees C. The K-m, V-max, and k(cat) values for lichenan are 2.96 mg/ml, 6,951 mu mol/min center dot mg, and 3,131 s(-1), respectively. For barley beta-glucan the values are 3.73 mg/ml, 8,939 mu mol/min center dot mg, and 4,026 s(-1), respectively. The recombinant Bg1 had resistance to pepsin and trypsin. Other features of recombinant Bg1 including temperature and pH stability, and sensitivity to some metal ions and chemical reagents were also characterized.