Yeast RNase III Triggers Polyadenylation-Independent Transcription Termination

被引：42
作者：
Ghazal, Ghada [1 ]
Gagnon, Jules [1 ]
Jacques, Pierre-Etienne [2 ]
Landry, Josette-Renee [2 ]
Robert, Francois [2 ,3 ]
Abou Elela, Sherif [1 ]
机构：
[1] Univ Sherbrooke, RNA Grp, Dept Microbiol & Infectiol, Fac Med & Sci Sante, Sherbrooke, PQ J1H 5N4, Canada
[2] Inst Rech Clin Montreal, Montreal, PQ H2W 1R7, Canada
[3] Univ Montreal, Dept Med, Fac Med, Montreal, PQ H3T 1J4, Canada
来源：
MOLECULAR CELL | 2009年 / 36卷 / 01期
基金：
加拿大健康研究院;
关键词：
PRE-RIBOSOMAL-RNA; MESSENGER-RNAS; POLYMERASE-II; SACCHAROMYCES-CEREVISIAE; GENE DELETION; PROTEIN; SNORNA; IDENTIFICATION; EXPRESSION; INTERACTS;
D O I：
10.1016/j.molcel.2009.07.029
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transcription termination of messenger RNA (mRNA) is normally achieved by polyadenylation followed by Rat1p-dependent 5'-3' exoribonuleolytic degradation of the downstream transcript. Here we show that the yeast ortholog of the dsRNA-specific ribonuclease III (Rnt1p) may trigger Rat1p-dependent termination of RNA transcripts that fail to terminate near polyadenylation signals. Rnt1p cleavage sites were found downstream of several genes, and the deletion of RNT1 resulted in transcription readthrough. Inactivation of Rat1p impaired Rnt1p-dependent termination and resulted in the accumulation of 3' end cleavage products. These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals.
引用
收藏
页码：99 / 109
页数：11
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