Ultrastructural localization of Trypanosoma cruzi lysosomes by aryl sulphatase cytochernistry

Lysosomes of trypanosomatid protozoa are poorly known. In this work we have cytochernically detected the lysosomal enzyme aryl sulphatase in the trypanosomatids Trypanosoma cruzi and Crithidiafasciculata, by using p-nitrocatecholsulph ate as substrate. Positive reaction was located exclusively inside membrane-bound cytoplasmic vesicles distributed throughout the cell body. Electron-dense reaction was either dispersed homogeneously through the vesicular matrix or located at the vesicle periphery, apposed to the membrane, with fine granular deposits occasionally found at the vesicular matrix. Trypomastigote and epimastigote forms of T cruzi lacked electron-dense deposits at the plasma membrane, thus indicating that aryl sulphatase was not secreted to the environment. Furthermore, no positive reaction was detected in epimastigote reservosomes, which are organelles considered as pre-lysosomal compartments. Thus, our data show that reservosomes and lysosomes are organelles that can be distinguished by the cytochemical localization of aryl sulphatase in T cruzi epimastigotes and trypomastigotes. Positive reaction in cytoplasmic vesicles of C fasciculata choanomastigotes confirmed the specificity of the reaction for lysosomes in other trypanosomatid species. (c) 2006 Elsevier Ltd. All rights reserved.