GLASS BEAD TRANSFORMATION METHOD FOR GRAM-POSITIVE BACTERIA

被引：0
|
作者：
Rattanachaikunsopon, Pongsak [1 ]
Phumkhachorn, Parichat [1 ]
机构：
[1] Ubon Ratchathani Univ, Fac Sci, Dept Biol Sci, Warin Chamrap 34190, Ubon Ratchathan, Thailand
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2009年 / 40卷 / 04期
关键词：
transformation; Gram-positive bacteria; glass bead; STREPTOCOCCUS-LACTIS PROTOPLASTS; PLASMID DNA; ELECTROPORATION PROTOCOL; LACTOBACILLUS; BACILLUS; STAPHYLOCOCCUS; OPTIMIZATION; ENTEROCOCCUS; LACTOCOCCUS; LEUCONOSTOC;
D O I：
10.1590/S1517-83822009000400025
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
引用
收藏
页码：923 / 926
页数：4
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