Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects

被引:9
作者
Khakpour, Golnaz [1 ]
Noruzinia, Mehrdad [2 ]
Izadi, Pantea [1 ]
Karami, Fatemeh [3 ]
Ahmadvand, Mohammad [4 ]
Heshmat, Ramin [5 ]
Amoli, Mahsa M. [6 ]
Tavakkoly-Bazzaz, Javad [1 ]
机构
[1] Univ Tehran Med Sci, Sch Med, Dept Med Genet, Tehran 1417613151, Iran
[2] Tarbiat Modares Univ, Dept Med Genet, Fac Med Sci, Tehran, Iran
[3] Islamic Azad Univ, Sci & Res Branch, Dept Med Genet, Tehran, Iran
[4] Univ Tehran Med Sci, Shariati Hosp, Hematol Oncol & Stem Cell Transplantat Res Ctr, Tehran, Iran
[5] Univ Tehran Med Sci, Endocrinol & Metab Populat Sci Inst, Chron Dis Res Ctr, Tehran, Iran
[6] Univ Tehran Med Sci, Endocrinol & Metab Mol Cellular Sci Inst, Metab Disorders Res Ctr, Tehran, Iran
关键词
White blood cells; methylation; microarray; breast cancer; BRCA1 PROMOTER METHYLATION; DNA METHYLATION; MICROSATELLITE INSTABILITY; TUMOR; GENES; RISK; EXPRESSION; CELLS; HYPERMETHYLATION; HYPOMETHYLATION;
D O I
10.1177/1010428317695040
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1, and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies.
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页数:11
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