Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects

被引:9
作者
Khakpour, Golnaz [1 ]
Noruzinia, Mehrdad [2 ]
Izadi, Pantea [1 ]
Karami, Fatemeh [3 ]
Ahmadvand, Mohammad [4 ]
Heshmat, Ramin [5 ]
Amoli, Mahsa M. [6 ]
Tavakkoly-Bazzaz, Javad [1 ]
机构
[1] Univ Tehran Med Sci, Sch Med, Dept Med Genet, Tehran 1417613151, Iran
[2] Tarbiat Modares Univ, Dept Med Genet, Fac Med Sci, Tehran, Iran
[3] Islamic Azad Univ, Sci & Res Branch, Dept Med Genet, Tehran, Iran
[4] Univ Tehran Med Sci, Shariati Hosp, Hematol Oncol & Stem Cell Transplantat Res Ctr, Tehran, Iran
[5] Univ Tehran Med Sci, Endocrinol & Metab Populat Sci Inst, Chron Dis Res Ctr, Tehran, Iran
[6] Univ Tehran Med Sci, Endocrinol & Metab Mol Cellular Sci Inst, Metab Disorders Res Ctr, Tehran, Iran
关键词
White blood cells; methylation; microarray; breast cancer; BRCA1 PROMOTER METHYLATION; DNA METHYLATION; MICROSATELLITE INSTABILITY; TUMOR; GENES; RISK; EXPRESSION; CELLS; HYPERMETHYLATION; HYPOMETHYLATION;
D O I
10.1177/1010428317695040
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1, and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies.
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页数:11
相关论文
共 72 条
[1]   Host Factors and Risk of Breast Cancer Recurrence: Genetic, Epigenetic and Biologic Factors and Breast Cancer Outcomes [J].
Ambrosone, Christine B. ;
Hong, Chi-Chen ;
Goodwin, Pamela J. .
IMPROVING OUTCOMES FOR BREAST CANCER SURVIVORS: PERSPECTIVES ON RESEARCH CHALLENGES AND OPPORTUNITIES, 2015, 862 :143-153
[2]   Characterization of human Rab20 overexpressed in exocrine pancreatic carcinoma [J].
Amillet, JM ;
Ferbus, D ;
Real, FX ;
Antony, C ;
Muleris, M ;
Gress, TM ;
Goubin, G .
HUMAN PATHOLOGY, 2006, 37 (03) :256-263
[3]   A BRCA1-mutation associated DNA methylation signature in blood cells predicts sporadic breast cancer incidence and survival [J].
Anjum, Shahzia ;
Fourkala, Evangelia-Ourania ;
Zikan, Michal ;
Wong, Andrew ;
Gentry-Maharaj, Aleksandra ;
Jones, Allison ;
Hardy, Rebecca ;
Cibula, David ;
Kuh, Diana ;
Jacobs, Ian J. ;
Teschendorff, Andrew E. ;
Menon, Usha ;
Widschwendter, Martin .
GENOME MEDICINE, 2014, 6
[4]   Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast [J].
Bae, Ja Seong ;
Choi, Jin Soo ;
Baik, Seung Ho ;
Park, Woo Chan ;
Song, Byung Joo ;
Kim, Jeong Soo ;
Lim, Young ;
Jung, Sang Seol .
WORLD JOURNAL OF SURGICAL ONCOLOGY, 2010, 8
[5]  
Bauer M, 2016, CLIN EPIGENETICS, V8, DOI [10.1186/s13148-016-0249-7, 10.1186/s13148-015-0113-1]
[6]   DNA methylation epigenotypes in breast cancer molecular subtypes [J].
Bediaga, Naiara G. ;
Acha-Sagredo, Amelia ;
Guerra, Isabel ;
Viguri, Amparo ;
Albaina, Carmen ;
Ruiz Diaz, Irune ;
Rezola, Ricardo ;
Jesus Alberdi, Maria ;
Dopazo, Joaquin ;
Montaner, David ;
de Renobales, Mertxe ;
Fernandez, Agustin F. ;
Field, John K. ;
Fraga, Mario F. ;
Liloglou, Triantafillos ;
de Pancorbo, Marian M. .
BREAST CANCER RESEARCH, 2010, 12 (05)
[7]   Epigenetic silencing and deletion of the BRCA1 gene in sporadic breast cancer [J].
Birgisdottir, Valgerdur ;
Stefansson, Olafur A. ;
Bodvarsdottir, Sigridur K. ;
Hilmarsdottir, Holmfridur ;
Jonasson, Jon G. ;
Eyfjord, Jorunn E. .
BREAST CANCER RESEARCH, 2006, 8 (04)
[8]   BRCA1 promoter methylation in peripheral blood DNA was identified in sporadic breast cancer and controls [J].
Bosviel, Remy ;
Garcia, Stephane ;
Lavediaux, Guillaume ;
Michard, Emilie ;
Dravers, Marine ;
Kwiatkowski, Fabrice ;
Bignon, Yves-Jean ;
Bernard-Gallon, Dominique J. .
CANCER EPIDEMIOLOGY, 2012, 36 (03) :E177-E182
[9]   Intragenic ATM Methylation in Peripheral Blood DNA as a Biomarker of Breast Cancer Risk [J].
Brennan, Kevin ;
Garcia-Closas, Montserrat ;
Orr, Nick ;
Fletcher, Olivia ;
Jones, Michael ;
Ashworth, Alan ;
Swerdlow, Anthony ;
Thorne, Heather ;
Riboli, Elio ;
Vineis, Paolo ;
Dorronsoro, Miren ;
Clavel-Chapelon, Francoise ;
Panico, Salvatore ;
Onland-Moret, N. Charlotte ;
Trichopoulos, Dimitrios ;
Kaaks, Rudolf ;
Khaw, Kay-Tee ;
Brown, Robert ;
Flanagan, James M. .
CANCER RESEARCH, 2012, 72 (09) :2304-2313
[10]   LINE-1 hypomethylation is associated with bladder cancer risk among nonsmoking Chinese [J].
Cash, Haley L. ;
Tao, Li ;
Yuan, Jian-Min ;
Marsit, Carmen J. ;
Houseman, E. Andres ;
Xiang, Yong-Bing ;
Gao, Yu-Tang ;
Nelson, Heather H. ;
Kelsey, Karl T. .
INTERNATIONAL JOURNAL OF CANCER, 2012, 130 (05) :1151-1159