The Aurora B Kinase and the Polycomb Protein Ring1B Combine to Regulate Active Promoters in Quiescent Lymphocytes

被引：91

作者：

Frangini, Alberto ^{[1
]}

Sjoeberg, Marcela ^{[1
]}

Roman-Trufero, Monica ^{[1
]}

Dharmalingam, Gopuraja ^{[1
]}

Haberle, Vanja ^{[2
]}

Bartke, Till ^{[3
]}

Lenhard, Boris ^{[2
]}

Malumbres, Marcos ^{[4
,5
]}

Vidal, Miguel ^{[6
]}

Dillon, Niall ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, MRC Clin Sci Ctr, Gene Regulat & Chromatin Grp, London W12 0NN, England

[2] Univ London Imperial Coll Sci Technol & Med, MRC Clin Sci Ctr, Computat Regulatory Genom Grp, London W12 0NN, England

[3] Univ London Imperial Coll Sci Technol & Med, MRC Clin Sci Ctr, Chromatin Biochem Grp, London W12 0NN, England

[4] Spanish Natl Canc Res Ctr CNIO, Cell Div, E-28029 Madrid, Spain

[5] Spanish Natl Canc Res Ctr CNIO, Canc Grp, E-28029 Madrid, Spain

[6] CSIC, Ctr Invest Biol, Madrid 28040, Spain

来源：

MOLECULAR CELL | 2013年 / 51卷 / 05期

基金：

英国医学研究理事会;

关键词：

CELL-CYCLE PROGRESSION; RNA-POLYMERASE-II; HISTONE H3; GENE-EXPRESSION; PHOSPHORYLATION; COMPLEX; H2A; ACTIVATION; CHROMATIN; UBIQUITINATION;

D O I：

10.1016/j.molcel.2013.08.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversible cellular quiescence is critical for developmental processes in metazoan organisms and is characterized by a reduction in cell size and transcriptional activity. We show that the Aurora B kinase and the polycomb protein Ring1B have essential roles in regulating transcriptionally active genes in quiescent lymphocytes. Ring1B and Aurora B bind to a wide range of active promoters in resting B and T cells. Conditional knockout of either protein results in reduced transcription and binding of RNA Pol II to promoter regions and decreased cell viability. Aurora B phosphorylates histone H3S28 at active promoters in resting B cells as well as inhibiting Ring1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes. Our results identify a mechanism for regulating transcription in quiescent cells that has implications for epigenetic regulation of the choice between proliferation and quiescence.

引用

页码：647 / 661

页数：15

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