Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger

被引：51
|
作者：
Aifa, MS [1 ]
Sayadi, S [1 ]
Gargouri, A [1 ]
机构：
[1] Ctr Biotechnol Sfax, Sfax, Tunisia
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 10期
关键词：
Aspergillus niger; heterologous expression; lignin peroxidase; Phanerochaete chrysosporium;
D O I：
10.1023/A:1005541206270
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The lignin peroxidase (isoenzyme H8) of Phanerochaete chrysosporium was expressed in Aspergillus niger under the control of plant nopaline synthase (NOS) promoter and terminator. H8 mRNA was produced in this heterologous system. Western blot analysis showed that the recombinant protein was of size similar to the native H8. The extracellular lignin peroxidase activity in these primary constructs was positive, though weak (1.12 nKat mg(-1)).
引用
收藏
页码：849 / 853
页数：5
相关论文
共 50 条
  • [1] Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger
    Mohamed Sami Aifa
    Sami Sayadi
    Ali Gargouri
    Biotechnology Letters, 1999, 21 : 849 - 853
  • [2] Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica
    Wang, HK
    Lu, FP
    Sun, WF
    Du, LX
    BIOTECHNOLOGY LETTERS, 2004, 26 (20) : 1569 - 1573
  • [3] Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica
    Haikuan Wang
    Fuping Lu
    Yafan Sun
    Lianxiang Du
    Biotechnology Letters, 2004, 26 : 1569 - 1573
  • [4] Cultivation of Phanerochaete chrysosporium and lignin peroxidase activity
    Kim, YK
    Kim, G
    Jeong, MS
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 6 (06) : 420 - 424
  • [5] PRODUCTION OF PHANEROCHAETE-CHRYSOSPORIUM LIGNIN PEROXIDASE
    LINKO, S
    BIOTECHNOLOGY ADVANCES, 1992, 10 (02) : 191 - 236
  • [6] Heterologous expression of a thermostable manganese peroxidase from Dichomitus squalens in Phanerochaete chrysosporium
    Li, DM
    Youngs, HL
    Gold, MH
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 385 (02) : 348 - 356
  • [7] Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
    Woo S.H.
    Cho J.S.
    Lee B.S.
    Kim E.K.
    Biotechnology and Bioprocess Engineering, 2004, 9 (4) : 256 - 260
  • [8] Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
    Woo, SH
    Cho, JS
    Lee, BS
    Kim, EK
    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2004, 9 (04) : 256 - 260
  • [9] Reaction of lignin peroxidase of Phanerochaete chrysosporium in organic solvents
    Yoshida, S
    Watanabe, T
    Honda, Y
    Kuwahara, M
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (04) : 711 - 713
  • [10] Lignin peroxidase production by Phanerochaete chrysosporium immobilized on polyurethane foam
    Nakamura, Y
    Sawada, T
    Sungusia, MG
    Kobayashi, F
    Kuwahara, M
    Ito, H
    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1997, 30 (01) : 1 - 6
12345