Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger

被引：51
|
作者：
Aifa, MS [1 ]
Sayadi, S [1 ]
Gargouri, A [1 ]
机构：
[1] Ctr Biotechnol Sfax, Sfax, Tunisia
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 10期
关键词：
Aspergillus niger; heterologous expression; lignin peroxidase; Phanerochaete chrysosporium;
D O I：
10.1023/A:1005541206270
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The lignin peroxidase (isoenzyme H8) of Phanerochaete chrysosporium was expressed in Aspergillus niger under the control of plant nopaline synthase (NOS) promoter and terminator. H8 mRNA was produced in this heterologous system. Western blot analysis showed that the recombinant protein was of size similar to the native H8. The extracellular lignin peroxidase activity in these primary constructs was positive, though weak (1.12 nKat mg(-1)).
引用
收藏
页码：849 / 853
页数：5
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