共 50 条
Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum
被引:316
作者:
BenSaad, Lamees A.
[1
]
Kim, Kah Hwi
[1
]
Quah, Chin Chew
[1
]
Kim, Wee Ric
[3
]
Shahimi, Mustafa
[2
]
机构:
[1] Malaysia Inst Med Res, Dept Physiol, Fac Med, Kuala Lumpur 50603, Malaysia
[2] Univ Coll Lincoln, Petaling Jaya 47301, Malaysia
[3] Malaysia Inst Med Res, Kuala Lumpur 50603, Malaysia
来源:
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
|
2017年
/
17卷
关键词:
Inflammation;
Punica granatum;
Cytotoxicity;
Cytokines;
Ellagic acid;
Gallic acid;
Punicalagin;
NITRIC-OXIDE;
PROSTAGLANDIN E-2;
POMEGRANATE;
L;
LIPOPOLYSACCHARIDE;
INFLAMMATION;
ANTIOXIDANT;
EXTRACT;
MACROPHAGES;
ACTIVATION;
D O I:
10.1186/s12906-017-1555-0
中图分类号:
R [医药、卫生];
学科分类号:
10 ;
摘要:
Background: Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammation give rise to using medicinal herbs as alternative therapies. This study aimed to evaluate the anti-inflammatory effect of isolated compounds from the ethyl acetate (EtOAc) fraction of P. granatum by determination of their inhibitory effects on lipopolysaccharide (LPS), stimulated nitric oxide (NO), prostaglandin E2 (PGE-2), interleukin-6 (IL-6) and cyclooxxgenase-2 (COX-2) release from RAW264.7 cells. Methods: The compounds ellagic acid, gallic acid and punicalagin A&B were isolated from EtOAc by high performance liquid chromatography (HPLC) and further identified by mass spectrometry (MS). The inhibitory effect of ellagic acid, gallic acid and punicalagin A&B were evaluated on the production of LPS-induced NO by Griess reagent, PGE-2 and IL-6 by immunoassay kit and prostaglandin E2 competitive ELISA kit, and COX-2 by Western blotting. Results: Ellagic acid, gallic acid and punicalagin A&B potentially inhibited LPS-induced NO, PGE-2 and IL-6 production. Conclusion: The results indicate that ellagic acid, gallic acid and punicalagin may be the compounds responsible for the anti-inflammatory potential of P. granatum.
引用
收藏
页数:10
相关论文
共 50 条