Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA

被引：21

作者：

Minami, Motoyasu ^{[1
]}

Nishio, Kaori ^{[1
]}

Ajioka, Yui ^{[1
]}

Kyushima, Hiroaki ^{[1
]}

Shigeki, Katsuki ^{[2
]}

Kinjo, Kaneo ^{[3
]}

Yamada, Kazuno ^{[4
]}

Nagai, Masashi ^{[4
]}

Satoh, Kiyoshi ^{[4
]}

Sakurai, Yozo ^{[5
]}

机构：

[1] Chubu Univ, Dept Environm Biol, Coll Biosci & Biotechnol, Aichi 4878501, Japan

[2] Natl Inst Biomed Innovat, Res Ctr Med Plant Resources, Tanegashima Div, Kagoshima 8913604, Japan

[3] Okinawa Agr Expt Stn, Hort Branch, Lab Root & Tuber Crop, Okinawa 9042241, Japan

[4] Kinjirushi Co Ltd, R&D Div, Nakagawa Ku, Aichi 4548526, Japan

[5] JICA, Aichi 4890911, Japan

来源：

JOURNAL OF NATURAL MEDICINES | 2009年 / 63卷 / 01期

关键词：

Chloroplast DNA; C; aromatica; longa; xanthorrhiza; zedoaria; NONCODING REGIONS; UNIVERSAL PRIMERS; AMPLIFICATION; GENE; SEQUENCE;

D O I：

10.1007/s11418-008-0283-7

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa.

引用

页码：75 / 79

页数：5

共 12 条

[1] A SET OF UNIVERSAL PRIMERS FOR AMPLIFICATION OF POLYMORPHIC NONCODING REGIONS OF MITOCHONDRIAL AND CHLOROPLAST DNA IN PLANTS
DEMESURE, B
SODZI, N
PETIT, RJ
[J]. MOLECULAR ECOLOGY, 1995, 4 (01) : 129 - 131
[2] TURMERIC - CHEMISTRY, TECHNOLOGY, AND QUALITY
GOVINDARAJAN, VS
[J]. CRC CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1980, 12 (03): : 199 - 301
[3] PHYLOGENETIC-RELATIONSHIPS OF FERNS DEDUCED FROM RBCL GENE SEQUENCE
HASEBE, M
ITO, M
KOFUJI, R
UEDA, K
IWATSUKI, K
[J]. JOURNAL OF MOLECULAR EVOLUTION, 1993, 37 (05) : 476 - 482
[4] Comparison of Curcuma sp in Yakushima with C-aeruginosa and C-zedoaria in Java']Java by trnK gene sequence, RAPD pattern and essential oil component
Kitamura, Chinami
Nagoe, Tetsuro
Prana, Made Sri
Agusta, Andria
Ohashi, Kazuyoshi
Shibuya, Hirotaka
[J]. JOURNAL OF NATURAL MEDICINES, 2007, 61 (03) : 239 - 243
[5] Mikage Masayuki, 2003, Natural Medicines, V57, P246
[6] Nishizawa T., 2000, Journal of Phytogeography Taxon, V48, P63, DOI [10.24517/00055283, DOI 10.24517/00055283]
[7] Rakhunde SD, 1998, J FOOD SCI TECH MYS, V35, P352
[8] Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication
Sasaki, Y
Fushimi, H
Cao, H
Cai, SQ
Komatsu, K
[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2002, 25 (12) : 1593 - 1599
[9] UNIVERSAL PRIMERS FOR AMPLIFICATION OF 3 NONCODING REGIONS OF CHLOROPLAST DNA
TABERLET, P
GIELLY, L
PAUTOU, G
BOUVET, J
[J]. PLANT MOLECULAR BIOLOGY, 1991, 17 (05) : 1105 - 1109
[10] Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes
Tsumura, Y
Kawahara, T
Wickneswari, R
Yoshimura, K
[J]. THEORETICAL AND APPLIED GENETICS, 1996, 93 (1-2) : 22 - 29

← 1 2 →