Screening, identification, and characterization of α-xylosidase from a soil metagenome

被引：20
作者：
Matsuzawa, Tomohiko [1 ]
Kimura, Nobutada [1 ]
Suenaga, Hikaru [1 ]
Yaoi, Katsuro [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba Cent 6,1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2016年 / 122卷 / 04期
关键词：
alpha-Xylosidase; Metagenome; GH31; Xyloglucan; Xylose; BETA-D-XYLOSIDASE; MOLECULAR CHARACTERIZATION; XYLOGLUCAN OLIGOSACCHARIDES; STRUCTURAL-ANALYSIS; TRICHODERMA-REESEI; SEQUENCE-ANALYSIS; CLONING; GENE; EXPRESSION; HYDROLASE;
D O I：
10.1016/j.jbiosc.2016.03.012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel alpha-xylosidase, MeXyl31, was isolated and characterized from a soil metagenomic library. The amino acid sequence of MeXyl31 showed a slight homology with other characterized alpha-xylosidases. The optimal pH and temperature of recombinant MeXyl31 were pH 5.5 and 45 degrees C, respectively. Recombinant MeXyl31 had a higher alpha-xylosidase activity toward pNP alpha-D-xylopyranoside than pNP alpha-D-glucopyranoside, isoprimeverose, and other xyloglucan oligosaccharides. The k(cat)/K-m value toward pNP alpha-D-xylopyranoside was about 750-fold higher than that of isoprimeverose. MeXyl31 activity was strongly inactivated in the presence of zinc and copper ions. MeXyl31 is the first alpha-xylosidase isolated from the metagenome and, relative to other xyloglucan oligosaccharides, shows higher activity toward pNP alpha-D-xylopyranoside. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：393 / 399
页数：7
相关论文
共 58 条
  • [1] The RAST server: Rapid annotations using subsystems technology
    Aziz, Ramy K.
    Bartels, Daniela
    Best, Aaron A.
    DeJongh, Matthew
    Disz, Terrence
    Edwards, Robert A.
    Formsma, Kevin
    Gerdes, Svetlana
    Glass, Elizabeth M.
    Kubal, Michael
    Meyer, Folker
    Olsen, Gary J.
    Olson, Robert
    Osterman, Andrei L.
    Overbeek, Ross A.
    McNeil, Leslie K.
    Paarmann, Daniel
    Paczian, Tobias
    Parrello, Bruce
    Pusch, Gordon D.
    Reich, Claudia
    Stevens, Rick
    Vassieva, Olga
    Vonstein, Veronika
    Wilke, Andreas
    Zagnitko, Olga
    [J]. BMC GENOMICS, 2008, 9 (1)
  • [2] Cloning and Characterization of Two β-Glucosidase/Xylosidase Enzymes from Yak Rumen Metagenome
    Bao, Lei
    Huang, Qiang
    Chang, Lei
    Sun, Qingwen
    Zhou, Jungang
    Lu, Hong
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 166 (01) : 72 - 86
  • [3] Cloning, expression, and characterization of an oligoxyloglucan reducing end-specific xyloglucanobiohydrolase from Aspergillus nidulans
    Bauer, S
    Vasu, P
    Mort, AJ
    Somerville, CR
    [J]. CARBOHYDRATE RESEARCH, 2005, 340 (17) : 2590 - 2597
  • [4] Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls
    Bauer, Stefan
    Vasu, Prasanna
    Persson, Staffan
    Mort, Andrew J.
    Somerville, Chris R.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (30) : 11417 - 11422
  • [5] The structure of an inverting GH43 β-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues
    Bruex, Christian
    Ben-David, Alon
    Shallom-Shezifi, Dalia
    Leon, Maya
    Niefind, Karsten
    Shoham, Gil
    Shoham, Yuval
    Schomburg, Dietmar
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2006, 359 (01) : 97 - 109
  • [6] Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus
    Chaillou, S
    Lokman, BC
    Leer, RJ
    Posthuma, C
    Postma, PW
    Pouwels, PH
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (09) : 2312 - 2320
  • [7] Molecular characterisation of a xyloglucan oligosaccharide-acting α-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant 'apoplastic' α-D-glucosidases
    Crombie, HJ
    Chengappa, S
    Jarman, C
    Sidebottom, C
    Reid, JSG
    [J]. PLANTA, 2002, 214 (03) : 406 - 413
  • [8] Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus
    DeBoy, Robert T.
    Mongodin, Emmanuel F.
    Fouts, Derrick E.
    Tailford, Louise E.
    Khouri, Hoda
    Emerson, Joanne B.
    Mohamoud, Yasmin
    Watkins, Kisha
    Henrissat, Bernard
    Gilbert, Harry J.
    Nelson, Karen E.
    [J]. JOURNAL OF BACTERIOLOGY, 2008, 190 (15) : 5455 - 5463
  • [9] Glycoside Hydrolases from a targeted Compost Metagenome, activity-screening and functional characterization
    Dougherty, Michael J.
    D'haeseleer, Patrik
    Hazen, Terry C.
    Simmons, Blake A.
    Adams, Paul D.
    Hadi, Masood Z.
    [J]. BMC BIOTECHNOLOGY, 2012, 12
  • [10] EDWARDS M, 1988, J BIOL CHEM, V263, P4333
123456