Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery

被引：60

作者：

Cao, Zhenbang ^{[1
,2
]}

Adnan, Nik Nil M. ^{[1
]}

Wang, Guoying ^{[3
]}

Rawal, Aditya ^{[4
]}

Shi, Bingyang ^{[3
]}

Liu, Ruizhe ^{[1
]}

Liang, Kang ^{[1
,2
]}

Zhao, Lingyun ^{[5
]}

Gooding, J. Justin ^{[2
,6
,7
]}

Boyer, Cyrille ^{[1
,2
]}

Gu, Zi ^{[1
,2
]}

机构：

[1] Univ New South Wales, Sch Chem Engn, Sydney, NSW, Australia

[2] Univ New South Wales, ACN, Sydney, NSW, Australia

[3] Macquarie Univ, Fac Med & Hlth Sci, Dept Biomed Sci, Sydney, NSW, Australia

[4] Univ New South Wales, Mark Wainwright Analyt Ctr, NMR Facil, Sydney, NSW, Australia

[5] Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Beijing, Peoples R China

[6] Univ New South Wales, Sch Chem, Sydney, NSW, Australia

[7] Univ New South Wales, ARC Ctr Excellence Convergent Bionano Sci & Techn, Sydney, NSW, Australia

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2018年 / 521卷

基金：

英国医学研究理事会;

关键词：

Layered double hydroxide; PEGylation; Nanoparticle; Stability; Non-specific protein adsorption; IN-VIVO; SIRNA DELIVERY; RELEASE; NANOCOMPOSITES; NANOHYBRIDS; NANOSHEETS;

D O I：

10.1016/j.jcis.2018.03.006

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Conjugating nanoparticles with polyethylene glycol (PEG) is a useful strategy to improve the colloidal and biological stability of nanoparticles. However, studies on PEGylation of two-dimensional layered double hydroxide (LDH) nanoparticles are very limited. The present work reported two functionalization approaches to synthesize PEG-conjugated LDH nanoparticles by introducing phosphonic acid terminated PEG before and after LDH aging. The successful PEGylation was confirmed and suggested to be via electrostatic interaction and a ligand exchange process. Different functionalization approaches resulted in different binding types of PEG on/in LDH nanoparticles. The PEG coating maintained the dispersity of LDH nanoparticles in water and saline with the feeding mass ratio of 1:1. Further colloidal stability tests of PEGylated LDHs revealed that the PEGylated LDH dispersity was affected by the feeding mass ratio of PEG/LDH, the molar weight of PEG and anions intercalated in the LDHs. In a test to determine the extent of non-specific protein adsorption, the PEGylation was effective at resisting non-specific bovine serum albumin adsorption on LDH nanoparticles with both functionalization methods investigated. Moreover, PEGylated LDH nanoparticles had no effect on cell viability up to 500 mu g/mL, and demonstrated enhanced cellular uptake in a SK-MEL-28 cell culture. The results in this work indicate that conjugating phosphonic acid-terminated PEG on LDH nanoparticles is a promising strategy to improve the colloidal and biological stability of LDHs for biomedical applications. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：242 / 251

页数：10

共 21 条

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Huang, ZhiGang
Liu, ZheShuo
Ci, LiQian
Liu, ZhePeng
Liu, Yu
Yan, XueYing
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