Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments

被引：76
作者：
Chen, SL
Wilson, DB
机构：
[1] CORNELL UNIV, BIOCHEM MOL & CELL BIOL SECT, ITHACA, NY 14853 USA
[2] CORNELL UNIV, INST COMPARAT & ENVIRONM TOXICOL, ITHACA, NY 14853 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 06期
关键词：
D O I：
10.1128/AEM.63.6.2442-2445.1997
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg2+-contaminated environments.
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收藏
页码：2442 / 2445
页数：4
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