Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams

被引：75

作者：

Moh, K. J. ^{[2
]}

Yuan, X. -C. ^{[1
]}

Bu, J. ^{[1
]}

Zhu, S. W. ^{[3
]}

Gao, Bruce Z. ^{[4
,5
]}

机构：

[1] Nankai Univ, Minist Educ China, Key Lab Optoelect Informat Sci & Technol, Inst Modern Opt, Tianjin 300071, Peoples R China

[2] Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore

[3] Tianjin Union Med Ctr, Tianjin 300121, Peoples R China

[4] Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA

[5] Clemson Univ, Ctr Opt Mat Sci & Engn Technol, Clemson, SC 29634 USA

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 25期

基金：

新加坡国家研究基金会; 中国国家自然科学基金;

关键词：

D O I：

10.1364/OE.16.020734

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map. (C) 2008 Optical Society of America

引用

页码：20734 / 20741

页数：8

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