Enteric viruses in water samples from Brazilian dairy farms

被引：14

作者：

de Oliveira, Lucas Kessler ^{[1
,2
]}

Fleck, Juliane Deise ^{[1
]}

Comerlato, Juliana ^{[3
]}

Kluge, Mariana ^{[1
]}

Bergamaschi, Bianca ^{[3
]}

Fabres, Rafael Bandeira ^{[1
]}

da Luz, Roger Bordin ^{[1
]}

dos Santos da Silva, Joseane Vanessa ^{[1
]}

Rodrigues, Manoela Tressoldi ^{[1
,2
]}

Genro, Joselaine Lino ^{[1
,2
]}

Staggemeier, Rodrigo ^{[1
,2
]}

Baldasso, Nelson ^{[4
]}

Spilki, Fernando Rosado ^{[1
,2
]}

机构：

[1] Univ Feevale, Inst Ciencias Saude, Mol Microbiol Lab, BR-93352000 Novo Hamburgo, RS, Brazil

[2] Univ Feevale, Programa Posgrad Qualidade Ambiental, BR-93352000 Novo Hamburgo, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Microbiol, Virol Lab, BR-90050170 Porto Alegre, RS, Brazil

[4] EMATER RS ASCAR, BR-90150053 Porto Alegre, RS, Brazil

来源：

AGRICULTURAL WATER MANAGEMENT | 2012年 / 111卷

关键词：

Virological analysis of water; Dairy; Enteric viruses; Environmental impact; Viral contamination of water; Precipitation; Surface water; Groundwater; MICROBIAL SOURCE-TRACKING; FECAL CONTAMINATION; BOVINE ENTEROVIRUS; CHILDREN; ROTAVIRUS; NOROVIRUS; PCR; PREVALENCE; PHOSPHORUS; INDICATORS;

D O I：

10.1016/j.agwat.2012.05.001

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The contamination of water by adenoviruses (AdV), rotaviruses (genogroup A. GARV). and enteroviruses (EV), including bovine enterovirus (BEV). was assessed for the first time in Brazilian dairy farms. The area of study, at the municipality of Taquara, is highly representative of the dairy farms in Southern Brazil: the farms are small, high animal densities per grazing area are used, the management of manure is poor and the sanitation for the human excreta is also deficient. Conventional polymerase chain reaction (PCR) was practiced to detect AdV, GARV, EV, and BEV in water samples taken from 27 collection sites in 10 dairy farms: two collections were made, after wet and dry weather conditions. For the first collection of water samples, during wet conditions posed by 18 consecutive days of rain prior to the sampling, 6 (22.2%) samples gave positive results for AdV and only 1 sample was positive for GARV. On the other hand, during the second collection, made under dry conditions, 4(16%) of the samples were positive for AdV, whereas 10(40%) samples gave positive results for GARV. EV was found on lower rates (9.6%) of the samples and BEV was not detected. Fecal contamination of water bodies is widespread on these farms, which could be confirmed either by detection of fecal coliforms and enteric viruses found. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：34 / 39

页数：6

共 31 条

[31] Symptomatic and Asymptomatic Infections of Rotavirus, Norovirus, and Adenovirus Among Hospitalized Children in Xi'an, China [J].

Zhang, Shuwan ;

Chen, Tsun-Hsuan ;

Wang, Juan ;

Dong, Changxin ;

Pan, Jingjing ;

Moe, Christine ;

Chen, Wei ;

Yang, Lihong ;

Wang, Xiaoqin ;

Tang, Helen ;

Li, Xu ;

Liu, Pengbo .

JOURNAL OF MEDICAL VIROLOGY, 2011, 83 (08) :1476-1484

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