The Optimization of Extraction of Cordycepin from Fruiting Body of Cordyceps militaris (L.) Link

被引：4
作者：
Zhang, Hong [2 ]
Wang, Jun-Wei [3 ]
Dong, Sheng-Zhong [3 ]
Xu, Fang-Xu [1 ]
Wang, Sheng-Hou [1 ]
机构：
[1] Shenyang Normal Univ, Res Inst Special Fungus, Shenyang 110034, Liaoning, Peoples R China
[2] Hebei United Univ, Coll Life Sci, Tangshan 063000, Hebei, Peoples R China
[3] Shenyang Normal Univ, Fac Engn, Shenyang 110034, Liaoning, Peoples R China
来源：
BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3 | 2012年 / 393-395卷
关键词：
Cordyceps militaris; cordycepin; optimization extraction; high-performance liquid chromatography (HPLC);
D O I：
10.4028/www.scientific.net/AMR.393-395.1024
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The optimization of extraction of cordycepin from fruiting body of Cordyceps militaris YCC-01 by water extraction, ethanol extraction, ultrasonic extraction, and synergistic extraction is studied in this paper. The optimal conditions, water extraction at 85 degrees C for 2.5h plus ultrasonic extraction at 600W for 35min, were determined through high performance liquid chromatography (HPLC). The dried fruiting body of cordycepin content was 9.559 mg/g by this synergistic extraction method. The yield was 66.2% higher than the control group 85 degrees C water extraction 2.5h and 11.3% higher than the room temperature ultrasonic extraction 35min. This method has a short extraction time, low cost, low loss of active ingredients and other characteristics with good prospects.
引用
收藏
页码：1024 / +
页数：2
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