Biosensors by means of the laser induced forward transfer technique

被引：15

作者：

Chatzipetrou, M. ^{[1
]}

Tsekenis, G. ^{[2
]}

Tsouti, V. ^{[3
]}

Chatzandroulis, S. ^{[3
]}

Zergioti, I. ^{[1
]}

机构：

[1] Natl Tech Univ Athens, Dept Phys, Athens 15780, Greece

[2] Acad Athens, Biomed Res Fdn, Athens 11527, Greece

[3] NCSR Demokritos, Inst Microelect, Athens 15310, Greece

来源：

APPLIED SURFACE SCIENCE | 2013年 / 278卷

关键词：

Laser printing; Oligonucleotides; Capacitance biosensor; CAPACITIVE BIOSENSOR; DNA HYBRIDIZATION; SURFACE; MICROARRAYS; DEPOSITION; STRESS; FILMS;

D O I：

10.1016/j.apsusc.2012.11.064

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This work presents the direct laser printing of thiol-modified oligonucleotides onto the surface of 3-glycidoxypropyltrimethoxysilane (GOPTS)-functionalized ultra-thin Low temperature oxide (LTO)/Si membranes for the fabrication of a label-free capacitive biosensor. During the hybridization of the target oligonucleotides with their immobilized complementary strands, various interaction forces develop, which contribute toward the deflection of the ultrathin LTO membranes, resulting in a capacitance shift. A number of different parameters were investigated for the laser printing conditions of the oligonucleotides that in turn affect the maximum deflection of the membranes and therefore the capacitance variation. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：250 / 254

页数：5

共 22 条

[1] Nanomechanics of the formation of DNA self-assembled monolayers and hybridization on microcantilevers [J].

Alvarez, M ;

Carrascosa, LG ;

Moreno, M ;

Calle, A ;

Zaballos, A ;

Lechuga, LM ;

Martínez-A, C ;

Tamayo, J .

LANGMUIR, 2004, 20 (22) :9663-9668

[2] Laser printing of single cells: Statistical analysis, cell viability, and stress [J].

Barron, JA ;

Krizman, DB ;

Ringeisen, BR .

ANNALS OF BIOMEDICAL ENGINEERING, 2005, 33 (02) :121-130

[3] METAL-DEPOSITION FROM A SUPPORTED METAL-FILM USING AN EXCIMER LASER [J].

BOHANDY, J ;

KIM, BF ;

ADRIAN, FJ .

JOURNAL OF APPLIED PHYSICS, 1986, 60 (04) :1538-1539

[4] Direct laser printing of biotin microarrays on low temperature oxide on Si substrates [J].

Boutopoulos, C. ;

Andreakou, P. ;

Kafetzopoulos, D. ;

Chatzandroulis, S. ;

Zergioti, I. .

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 2008, 205 (11) :2505-2508

[5] Liquid phase direct laser printing of polymers for chemical sensing applications [J].

Boutopoulos, Christos ;

Tsouti, Vasiliki ;

Goustouridis, Dimitrios ;

Chatzandroulis, Stavros ;

Zergioti, Ioanna .

APPLIED PHYSICS LETTERS, 2008, 93 (19)

[6]

Brown M.S., 2012, J FLUID MECH, P1

[7] Time-resolved dynamics of laser-induced micro-jets from thin liquid films [J].

Brown, Matthew S. ;

Kattamis, Nicholas T. ;

Arnold, Craig B. .

MICROFLUIDICS AND NANOFLUIDICS, 2011, 11 (02) :199-207

[8]

Chatzipetrou M., 2011, P EUR 25

[9] Laser-induced forward transfer of liquids:: Study of the droplet ejection process [J].

Colina, M. ;

Duocastella, M. ;

Fernandez-Pradas, J. M. ;

Serra, P. ;

Morenza, J. L. .

JOURNAL OF APPLIED PHYSICS, 2006, 99 (08)

[10] DNA deposition through laser induced forward transfer [J].

Colina, M ;

Serra, P ;

Fernández-Pradas, JM ;

Sevilla, L ;

Morenza, JL .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (08) :1638-1642

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