Synthesis and Application of an Efficient and Sensitive DNA Fluorescent Probe

被引：1

作者：

Wu Tong ^{[1
]}

Peng Xiao-Jun ^{[1
]}

Hu Ming-Ming ^{[1
]}

Liu Fei ^{[1
]}

Fan Jiang-Li ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2012年 / 33卷 / 07期

关键词：

Thiazole orange dimmer; Fluorescent probe; DNA quantitative detection; Live-cell fluorescence imaging; MINOR-GROOVE BINDER; LIVING CELLS; DYE; CYANINES; SINGLE; COLOR;

D O I：

10.3969/j.issn.0251-0790.2012.07.008

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel thiazole orange dimmer derivative Bi-TO3 was developed. The interaction of Bi-TO3 with DNA was investigated through the absorption, emission, circular dichroism spectroscopy and live-cell imaging experiments. As a specific probe for DNA, Bi-TO3 itself was almost non-fluorescent (quantum yield < 0.001%) in buffer. Upon binding to DNA, the fluorescence of Bi-TO3 increased approximately 950-fold, even without interferences of responding to RNA, BSA, viscosity, etc. Absorption and circular dichroism spectra showed that Bi-TO3 associated with DNA in a minor groove binding mode. The emission detection limit was 13.3 ng/mL, which manifested that Bi-TO3 could detect trace DNA in solution. Moreover, Bi-TO3 could be applied for live-cell fluorescence imaging with relatively low concentrations of 6-12 mu mol/L.

引用

页码：1407 / 1412

页数：6

共 24 条

[1] Bink K, 2001, HISTOCHEM CELL BIOL, V115, P293
[2] Color and constitution V The absorption of unsymmetrical cyanines resonance as a basis for a classification of dyes
Brooker, LGS
Keyes, GH
Williams, WW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1942, 64 : 199 - 210
[3] CHEN Ying-Jie, 2009, SICHUAN J ANATOMY, V17, P17
[4] Gao YH, 2010, CHEM J CHINESE U, V31, P2360
[5] Gill MR, 2009, NAT CHEM, V1, P662, DOI [10.1038/NCHEM.406, 10.1038/nchem.406]
[6] [顾晓怡 GU Xiao-Yi], 2009, [第四军医大学学报, Journal of the Fourth Military Medical University], V30, P1321
[7] DNA-templated assembly of helical cyanine dye aggregates: A supramolecular chain polymerization
Hannah, KC
Armitage, BA
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 2004, 37 (11) : 845 - 853
[8] Haugland R. P., 2005, HDB GUIDE FLUORESCEN
[9] Kashida H., 2004, ANGEW CHEM, V116, P6684
[10] Analytical Ancestry: "Firsts" in Fluorescent Labeling of Nucleosides, Nucleotides, and Nucleic Acids
Kricka, Larry J.
Fortina, Paolo
[J]. CLINICAL CHEMISTRY, 2009, 55 (04) : 670 - 683

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