Assessment of Molecular Interactions through Magnetic Relaxation

被引：10

作者：

Santiesteban, Oscar J. ^{[1
]}

Kaittanis, Charalambos ^{[1
]}

Perez, J. Manuel ^{[1
]}

机构：

[1] Univ Cent Florida, Nanosci Technol Ctr, Dept Chem, Orlando, FL 32826 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 27期

关键词：

dissociation constants; magnetic nanoparticles; membrane receptors; protein-ligand interactions; protein-protein interactions; SUPERPARAMAGNETIC IRON-OXIDE; PROTEIN; IDENTIFICATION; BIND;

D O I：

10.1002/anie.201202077

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Just relax: The dissociation constants (KD) of various molecular interactions can be determined using a novel competition assay with binding magnetic relaxation nanosensors. In this assay, changes in the magnetic relaxation (MR) of an aqueous suspension of the nanosensors facilitate the fast determination of KD values by using nanomolar protein concentrations (see picture). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：6728 / 6732

页数：5

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