Immobilization of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes of opposite charges for the development of the reusable fluorescent Cu2+-sensor

被引：46

作者：

Su, Lei ^{[1
]}

Shu, Tong ^{[1
]}

Wang, Zongwei ^{[1
]}

Cheng, Jingya ^{[1
]}

Xue, Feng ^{[1
]}

Li, Chenzhong ^{[2
]}

Zhang, Xueji ^{[1
]}

机构：

[1] Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China

[2] Florida Int Univ, Coll Engn & Comp, Miami, FL 33174 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 44卷

关键词：

Gold nanoclusters; Polyelectrolyte; Fluorescence; Reusable sensor; Copper ion; AU NANOCLUSTERS; GLUCOSE-OXIDASE; WATER; COMPLEXES; ELECTRODE; MEMBRANE; IONS;

D O I：

10.1016/j.bios.2013.01.005

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, we reported a facile method of fabricating a reusable fluorescent Cu2+-sensor. To fabricate the reusable sensor, the bovine serum albumin-protected gold nanoclusters (BSAGNCs) were complexed with polyelectrolytes, i.e., positively charged polydiallyldimethylammonium (PDDA) and negatively charged polystyrenesulfonate (PSS), and were coated on a glass slide. The prepared film-modified glass slides exhibited the recyclability of fluorescent signal "off-on" behaviors: the fluorescence could be switched "off" by immersing the film sensors in Cu2+ solution and "on" by washing with ethylene diamine tetraacetic acid (EDTA) solution. The prepared film-modified glass slides exhibited high selectivity towards Cu2+ the fluorescence quenching behavior in the form of the Stern-Volmer equation. In addition, the spiked tap water samples were analyzed with satisfactory results. These demonstrations provide a new convenient approach to develop the BSAGNCs-based, reusable fluorescence sensors. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：16 / 20

页数：5

共 28 条

[1] Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate [J].

Chen, Zhanguang ;

Qian, Sihua ;

Chen, Xi ;

Gao, Wenhua ;

Lin, Yuejuan .

ANALYST, 2012, 137 (18) :4356-4361

[2] Protein-templated gold nanoclusters based sensor for off-on detection of ciprofloxacin with a high selectivity [J].

Chen, Zhanguang ;

Qian, Sihua ;

Chen, Junhui ;

Cai, Jie ;

Wu, Shuyan ;

Cai, Ziping .

TALANTA, 2012, 94 :240-245

[3] Response of polyelectrolyte complexes to subsequent addition of salts with different cations [J].

Dautzenberg, H ;

Kriz, J .

LANGMUIR, 2003, 19 (13) :5204-5211

[4] Etching colloidal gold nanocrystals with hyperbranched and multivalent polymers: A new route to fluorescent and water-soluble atomic clusters [J].

Duan, Hongwei ;

Nie, Shuming .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (09) :2412-+

[5] Fluorescent gold clusters as nanosensors for copper ions in live cells [J].

Durgadas, C. V. ;

Sharma, C. P. ;

Sreenivasan, K. .

ANALYST, 2011, 136 (05) :933-940

[6] Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion [J].

Guo, Weiwei ;

Yuan, Jipei ;

Wang, Erkang .

CHEMICAL COMMUNICATIONS, 2009, (23) :3395-3397

[7] Highly selective fluorescent sensors for Hg2+ based on bovine serum albumin-capped gold nanoclusters [J].

Hu, Dehong ;

Sheng, Zonghai ;

Gong, Ping ;

Zhang, Pengfei ;

Cai, Lintao .

ANALYST, 2010, 135 (06) :1411-1416

[8] Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose [J].

Jin, Lihua ;

Shang, Li ;

Guo, Shaojun ;

Fang, Youxing ;

Wen, Dan ;

Wang, Li ;

Yin, Jianyuan ;

Dong, Shaojun .

BIOSENSORS & BIOELECTRONICS, 2011, 26 (05) :1965-1969

[9] PREPARATION AND AMPEROMETRIC GLUCOSE SENSITIVITY OF COVALENTLY BOUND GLUCOSE-OXIDASE TO (2-AMINOETHYL)FERROCENE ON AN AU ELECTRODE [J].

KUWABATA, S ;

OKAMOTO, T ;

KAJIYA, Y ;

YONEYAMA, H .

ANALYTICAL CHEMISTRY, 1995, 67 (10) :1684-1690

[10] Formation of Fluorescent Metal (Au, Ag) Nanoclusters Capped in Bovine Serum Albumin Followed by Fluorescence and Spectroscopy [J].

Le Guevel, Xavier ;

Hoetzer, Benjamin ;

Jung, Gregor ;

Hollemeyer, Klaus ;

Trouillet, Vanessa ;

Schneider, Marc .

JOURNAL OF PHYSICAL CHEMISTRY C, 2011, 115 (22) :10955-10963

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