Application of the inhibition of bacterial bioluminescence test for assessment of toxicity of carbon-based nanomaterials

被引：14
作者：
Deryabin, D. G. [1 ]
Aleshina, E. S. [1 ]
Efremova, L. V. [1 ]
机构：
[1] Orenburg State Univ, Orenburg, Russia
来源：
MICROBIOLOGY | 2012年 / 81卷 / 04期
关键词：
carbon-based nanomaterials; toxicity; biosensors; bioluminescence; E; coli; NANOTUBES;
D O I：
10.1134/S0026261712040042
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A method for assessment of integral biological toxicity of carbon-based nanomaterials (CBN) using a bacterial luminescent biosensor was developed, which accounted for the properties of the objects under study. The proposed approach includes a special procedure for the preparation of the analyzed CBN samples aimed at obtaining highly dispersed suspensions, an extended period of dynamic monitoring of the luminescence of the contacting sensor microorganism, and the special algorithm for quantification of the results of the bioluminescence analysis, in order to exclude the effect of the optical properties of CBN on the results of investigation. This method was used for assessment of the biological toxicity of a broad spectrum of CBN, such as single- and multi-walled nanotubes, nanofibres, and C60- and C70-fullerenes, in order to compare them in this respect to amorphous (nonstructured) carbon.
引用
收藏
页码：492 / 497
页数：6
相关论文
共 16 条
[1]  
Aleshina E.S., 2010, P ORENBURG U, P123
[2]  
[Anonymous], 2010, 12263410 FED CTR HYG
[3]   Inactivation of Bacterial Pathogens by Carbon Nanotubes in Suspensions [J].
Arias, L. Renea ;
Yang, Liju .
LANGMUIR, 2009, 25 (05) :3003-3012
[4]   Microbial whole-cell sensing systems of environmental pollutants [J].
Belkin, S .
CURRENT OPINION IN MICROBIOLOGY, 2003, 6 (03) :206-212
[5]  
Danilov V.S., 2002, MOS U B 16
[6]   Acute toxicity of carbon-based nanomaterials to Escherichia coli is partially dependent on the presence of process impurities [J].
Deryabin D.G. ;
Aleshina E.S. ;
Tlyagulova A.S. .
Nanotechnologies in Russia, 2011, 6 (7-8) :528-534
[7]   An investigation into the interaction between carbon-based nanomaterials and Escherichia coli cells using atomic force microscopy [J].
Deryabin D.G. ;
Vasilchenko A.S. ;
Aleshina E.S. ;
Tlyagulova A.S. ;
Nikiyan H.N. .
Nanotechnologies in Russia, 2010, 5 (11) :857-863
[8]  
Deryabin D. G., 2009, BAKTERIALNAYA BIOLYU
[9]   Toxicology of carbon nanomaterials: Status, trends, and perspectives on the special issue [J].
Hurt, RH ;
Monthioux, M ;
Kane, A .
CARBON, 2006, 44 (06) :1028-1033
[10]   REGRESSION AND CLUSTER-ANALYSIS OF THE ACUTE TOXICITY OF 267 CHEMICALS TO 6 SPECIES OF BIOTA AND THE OCTANOL WATER PARTITION-COEFFICIENT [J].
KAISER, KLE ;
ESTERBY, SR .
SCIENCE OF THE TOTAL ENVIRONMENT, 1991, 109 :499-514
12