Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins

被引：99
作者：
Bermel, W
Bertini, I [1 ]
Felli, IC
Kümmerle, R
Pierattelli, R
机构：
[1] Univ Florence, Magnet Resonance Ctr, I-50121 Florence, Italy
[2] Univ Florence, Dept Chem, I-50121 Florence, Italy
[3] Bruker BioSpin GmbH, Rheinstetten, Germany
[4] Bruker BioSpin GmbH, Fallanden, Switzerland
来源：
JOURNAL OF MAGNETIC RESONANCE | 2006年 / 178卷 / 01期
关键词：
protonless NMR; C-13; NMR; C-13-direct detection; sequential assignment; protein structure;
D O I：
10.1016/j.jmr.2005.08.011
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：56 / 64
页数：9
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