Spectrofluorimetric quantification of bilirubin using yttrium-norfloxacin complex as a fluorescence probe in serum samples

被引:27
作者
Kamruzzaman, Mohammad [1 ]
Alam, Al-Mahmnur [1 ]
Lee, Sang Hak [1 ]
Kim, Young Ho [2 ]
Kim, Gyu-Man [3 ]
Oh, Sang Hyub [4 ]
机构
[1] Kyungpook Natl Univ, Dept Chem, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Res Inst Adv Energy Technol, Taegu 702701, South Korea
[3] Kyungpook Natl Univ, Sch Mech Engn, Taegu 702701, South Korea
[4] Korea Res Inst Stand & Sci, Ctr Gas Anal, Taejon 305600, South Korea
基金
新加坡国家研究基金会;
关键词
Fluorescence; Norfloxacin; Yttrium; Bilirubin; Serum; QUANTITATIVE-DETERMINATION; CAPILLARY-ELECTROPHORESIS; ENZYMATIC METHOD; CHEMILUMINESCENCE; ALBUMIN; FRACTIONS; ACCURATE; ASSAY;
D O I
10.1016/j.jlumin.2012.06.013
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A simple and sensitive spectrofluorimetric method was developed to determine trace amounts of bilirubin (BR) using yttrium (Y3+)-norfloxacin (NFLX) complex as a fluorescence (FL) probe. NFLX can form a stable binary complex with Y3+ and markedly enhances the weak FL signal of the NFLX. The FL intensity of the Y3+-NFLX complex decreased significantly in the presence of BR in a buffer solution at pH=7.2. Under optimal conditions, the FL intensity decreased according to the BR concentration and showed a good linear relationship in the range of 0.03-2.3 mu g mL(-1) of BR with a correlation coefficient of 0.9988. The limit of detection for the determination of BR was 2.8 ng mL(-1) with a relative standard deviation (RSD) of 1.55% for five replicate determination of 0.05 mu g mL(-1) BR. The presented method offers higher sensitivity with simple instrumentation and was applied successfully in detecting BR at low concentrations. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:3053 / 3057
页数:5
相关论文
共 30 条
[11]   Study of the interaction between terazosin and serum albumin - Synchronous fluorescence determination of terazosin [J].
Jiang, CQ ;
Gao, MX ;
He, JX .
ANALYTICA CHIMICA ACTA, 2002, 452 (02) :185-189
[12]  
Kazmierczak SC, 2002, CLIN CHEM, V48, P1096
[13]  
Klemm J, 2000, ELECTROANAL, V12, P292, DOI 10.1002/(SICI)1521-4109(20000301)12:4<292::AID-ELAN292>3.0.CO
[14]  
2-3
[15]   ENZYMATIC DETERMINATION OF BILIRUBIN FRACTIONS IN SERUM [J].
KOSAKA, A ;
YAMAMOTO, C ;
MORISHITA, Y ;
NAKANE, K .
CLINICAL BIOCHEMISTRY, 1987, 20 (06) :451-458
[16]   A new enzymatic assay for selectively measuring conjugated bilirubin concentration in serum with use of bilirubin oxidase [J].
Kurosaka, K ;
Senba, S ;
Tsubota, H ;
Kondo, H .
CLINICA CHIMICA ACTA, 1998, 269 (02) :125-136
[17]  
LAKOWICZ JR, 1983, PRINCIPLES FLUORESCE, P359
[18]   Accurate direct spectrophotometric bilirubin measurement combined with blood gas analysis [J].
Laterza, OF ;
Smith, CH ;
Wilhite, TR ;
Landt, M .
CLINICA CHIMICA ACTA, 2002, 323 (1-2) :115-120
[19]   Quantitative determination of bilirubin by inhibition of chemiluminescence from lucigenin [J].
Lee, Hyun Sook ;
Karim, Mohammad Mainul ;
Alam, Seikh Mafiz ;
Lee, Sang Hak .
LUMINESCENCE, 2007, 22 (04) :331-337
[20]  
Liu J. W., 1998, CHIN J CHROMATOGR, V16