Spectrofluorimetric quantification of bilirubin using yttrium-norfloxacin complex as a fluorescence probe in serum samples

被引：27

作者：

Kamruzzaman, Mohammad ^{[1
]}

Alam, Al-Mahmnur ^{[1
]}

Lee, Sang Hak ^{[1
]}

Kim, Young Ho ^{[2
]}

Kim, Gyu-Man ^{[3
]}

Oh, Sang Hyub ^{[4
]}

机构：

[1] Kyungpook Natl Univ, Dept Chem, Taegu 702701, South Korea

[2] Kyungpook Natl Univ, Res Inst Adv Energy Technol, Taegu 702701, South Korea

[3] Kyungpook Natl Univ, Sch Mech Engn, Taegu 702701, South Korea

[4] Korea Res Inst Stand & Sci, Ctr Gas Anal, Taejon 305600, South Korea

来源：

JOURNAL OF LUMINESCENCE | 2012年 / 132卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

Fluorescence; Norfloxacin; Yttrium; Bilirubin; Serum; QUANTITATIVE-DETERMINATION; CAPILLARY-ELECTROPHORESIS; ENZYMATIC METHOD; CHEMILUMINESCENCE; ALBUMIN; FRACTIONS; ACCURATE; ASSAY;

D O I：

10.1016/j.jlumin.2012.06.013

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A simple and sensitive spectrofluorimetric method was developed to determine trace amounts of bilirubin (BR) using yttrium (Y3+)-norfloxacin (NFLX) complex as a fluorescence (FL) probe. NFLX can form a stable binary complex with Y3+ and markedly enhances the weak FL signal of the NFLX. The FL intensity of the Y3+-NFLX complex decreased significantly in the presence of BR in a buffer solution at pH=7.2. Under optimal conditions, the FL intensity decreased according to the BR concentration and showed a good linear relationship in the range of 0.03-2.3 mu g mL(-1) of BR with a correlation coefficient of 0.9988. The limit of detection for the determination of BR was 2.8 ng mL(-1) with a relative standard deviation (RSD) of 1.55% for five replicate determination of 0.05 mu g mL(-1) BR. The presented method offers higher sensitivity with simple instrumentation and was applied successfully in detecting BR at low concentrations. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：3053 / 3057

页数：5

共 30 条

[1] Measurement of unbound bilirubin by the peroxidase test using Zone Fluidics [J].