Hyperspectral Imaging of Lipids using Near-infrared Super Continuum Light

Lipids can be detected by infrared (IR) spectroscopy because lipids have significant absorption peaks derived from C-H or C=O vibration mode. Near infrared (NIR) spectroscopy can also give the information of absorptions. The significant absorption peaks are often observed by overtone of molecular vibration of mid-infrared (MIR) region. Hyperspectral imaging technology realizes to show us the spatial and chemical information. NIR hyperspectral imaging can show the distribution of chemical compounds by vibrational information. In this study, we developed the hyperspectral imaging system using NIR super continuum (SC) light to achieve a high contrast image. In this paper, we showed the hyperspectral imaging of lipid, triacylgrygrycerol under a skin. As the result, specific peak was probed at the wavelength of 1490, 1730 nm, and the distribution of lipid (triacylgrycerol) was confirmed from the spectral calculation. Therefore, Hyperspectral imaging with NIR SC light realized to probe the specific absorption peak and spatial distribution of lipids, triacylgrycerol.