(IscS-IscU)2 Complex Structures Provide Insights into Fe2S2 Biogenesis and Transfer

被引：121

作者：

Marinoni, Elodie N. ^{[1
]}

de Oliveira, Jaim S. ^{[2
]}

Nicolet, Yvain ^{[1
]}

Raulfs, Estella C. ^{[2
]}

Amara, Patricia ^{[1
]}

Dean, Dennis R. ^{[2
]}

Fontecilla-Camps, Juan C. ^{[1
]}

机构：

[1] Univ Grenoble 1, CNRS, Metalloprot Unit, Inst Biol Struct JP Ebel Commissariat Energie Ato, F-38027 Grenoble, France

[2] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 22期

基金：

美国国家科学基金会;

关键词：

biogenesis; computational chemistry; iron-sulfur clusters; scaffold proteins; IRON-SULFUR CLUSTERS; CYSTEINE DESULFURASE; CRYSTAL-STRUCTURE; ISCU; PROTEIN; SCAFFOLD; BIOSYNTHESIS; 2FE-2S; IDENTIFICATION; INTERMEDIATE;

D O I：

10.1002/anie.201201708

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron-sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe 2S 2-(IscS-IscU D35A)) 2 complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine (see picture). In air-exposed crystals the cluster is oxidized to an Fe 2S-S center; D35 is essential for complex dissociation. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5439 / 5442

页数：4

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